RRC ID 41674
Author Yageta M, Kuramochi M, Masuda M, Fukami T, Fukuhara H, Maruyama T, Shibuya M, Murakami Y.
Title Direct association of TSLC1 and DAL-1, two distinct tumor suppressor proteins in lung cancer.
Journal Cancer Res
Abstract The tumor suppressor gene TSLC1, which we recently identified in human non-small cell lung cancer, encodes a membrane glycoprotein of the immunoglobulin superfamily. Here, we report that TSLC1 directly associates with DAL-1, a gene product of another lung tumor suppressor belonging to the protein 4.1 family. TSLC1 additionally interacts with the actin filament through DAL-1 at the cell-cell attached site where the complex formation of TSLC1 and DAL-1 is dependent on the integrity of actin cytoskeleton. Redistribution of both TSLC1 and DAL-1 to the newly generated membrane ruffling areas suggests that these proteins are also involved in cell motility accompanying the actin rearrangement. Furthermore, restoration of TSLC1 expression strongly suppressed the metastasis of a human non-small cell lung cancer cell line, A549, from the spleen to the liver in nude mice. These findings, together with frequent loss of their expression in lung cancers, suggest that TSLC1 and DAL-1 play a critical role in the same pathway involved in the suppression of lung tumor formation and metastasis.
Volume 62(18)
Pages 5129-33
Published 2002-9-15
PMID 12234973
MeSH Actins / metabolism Amino Acid Sequence Animals COS Cells Carcinoma, Non-Small-Cell Lung / metabolism* Carcinoma, Non-Small-Cell Lung / pathology Cell Adhesion / physiology Cell Adhesion Molecule-1 Cell Adhesion Molecules Cytochalasin D / pharmacology Cytoskeleton / drug effects Cytoskeleton / metabolism Cytoskeleton / physiology Humans Immunoglobulins* Lung Neoplasms / metabolism* Lung Neoplasms / pathology Membrane Proteins / genetics Membrane Proteins / metabolism* Microfilament Proteins Microscopy, Fluorescence Molecular Sequence Data Protein Structure, Tertiary Proteins / genetics Proteins / metabolism* Sequence Homology, Amino Acid Tumor Cells, Cultured Tumor Suppressor Proteins / genetics Tumor Suppressor Proteins / metabolism*
IF 9.727
Times Cited 146
Human and Animal Cells