論文 - 詳細
| RRC ID | 41693 |
|---|---|
| 著者 | Ikeguchi M, Ueta T, Yamane Y, Hirooka Y, Kaibara N. |
| タイトル | Inducible nitric oxide synthase and survivin messenger RNA expression in hepatocellular carcinoma. |
| ジャーナル | Clin Cancer Res |
| Abstract |
PURPOSE:Proliferative activity and suppression of apoptosis of cancer cells are important to tumor progression in hepatocellular carcinoma (HCC). Recently, the expressions of inducible nitric oxide synthase (iNOS) and survivin mRNA have been reported to correlate with suppression of apoptosis in some tumors. However, the clinical importance of expression of these genes in HCC progression remains unclear. In the present study, the correlation between the expression of iNOS and survivin mRNA and the occurrence of spontaneous apoptosis and proliferative activity of cancer cells and prognostic importance of expression of these genes in HCC were investigated. EXPERIMENTAL DESIGN:Tissues were obtained by surgical resection of livers from 61 patients with HCC and 8 without HCC. Expressions of iNOS and survivin mRNA were evaluated using the reverse transcription-PCR in 61 tumors, 61 adjacent histologically noncancerous livers, and 8 normal livers. Apoptotic cancer cells and the proliferative activity of cancer cells were detected by immunohistochemistry. RESULTS:iNOS mRNA expression was detected in 34 of 61 (55.7%) HCCs, 19 of 61 (31.1%) noncancerous liver tissues adjacent to carcinoma, and none of the 8 normal livers. In addition, survivin mRNA was detected in 19 of 61 (31.1%) HCCs, none of 61 noncancerous liver tissues, and none of the 8 normal livers. iNOS mRNA expression did not correlate with the proliferative activity of cancer cells or with the occurrence of apoptosis in HCCs. In contrast, survivin mRNA expression strongly correlated with a high proliferative activity of cancer cells and a low apoptotic index. Disease-specific survivals did not differ between patients with iNOS-positive or -negative HCCs. Although, the disease-specific survival of patients with survivin-positive HCCs was significantly poorer than that of patients with survivin-negative HCCs. CONCLUSIONS:These results indicate that iNOS may not correlate with cancer cell-proliferative activity or apoptosis; survivin, however, may not only suppress apoptosis but also accelerate cancer cell-proliferative activity and play an important role in tumor progression in HCC. |
| 巻・号 | 8(10) |
| ページ | 3131-6 |
| 公開日 | 2002-10-1 |
| PMID | 12374680 |
| MeSH | Adult Aged Aged, 80 and over Apoptosis Carcinoma, Hepatocellular / genetics Carcinoma, Hepatocellular / metabolism* Carcinoma, Hepatocellular / pathology Cell Division DNA Primers / chemistry Female Humans Immunoenzyme Techniques Inhibitor of Apoptosis Proteins Ki-67 Antigen / metabolism Liver Diseases / genetics Liver Diseases / metabolism Liver Neoplasms / genetics Liver Neoplasms / metabolism* Liver Neoplasms / pathology Male Microtubule-Associated Proteins / genetics* Microtubule-Associated Proteins / metabolism Middle Aged Neoplasm Proteins Neoplasm Staging Nitric Oxide Synthase / genetics* Nitric Oxide Synthase / metabolism Nitric Oxide Synthase Type II RNA, Messenger / metabolism* RNA, Neoplasm / analysis Reverse Transcriptase Polymerase Chain Reaction Survival Rate Survivin Tumor Cells, Cultured |
| IF | 10.107 |
| 引用数 | 65 |
| WOS 分野 | ONCOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | |