| RRC ID |
41733
|
| Author |
Kanamori M, Kai C, Hayashizaki Y, Suzuki H.
|
| Title |
NF-kappaB activator Act1 associates with IL-1/Toll pathway adaptor molecule TRAF6.
|
| Journal |
FEBS Lett
|
| Abstract |
NF-kappaB activator 1 (Act1), also called CIKS, is a recently identified protein with NF-kappaB and AP-1 activation activities through its association with the IkappaB kinase complex. We identified and confirmed that Act1 interacts with tumor necrosis factor receptor-associated factor 6 (TRAF6); notably, Act1 binds to TRAF6 only among TRAF family proteins. The amino-terminal half of Act1 is required for its interaction with the TRAF domain. Act1-mediated NF-kappaB activation was inhibited by a dominant-negative mutant of TRAF6 in a dose-dependent manner, and IL-1-induced NF-kappaB activation was inhibited by a high level of Act1 expression. Our results suggest that Act1 is involved in IL-1/Toll-mediated signaling through TRAF6.
|
| Volume |
532(1-2)
|
| Pages |
241-6
|
| Published |
2002-12-4
|
| DOI |
10.1016/s0014-5793(02)03688-8
|
| PII |
S0014579302036888
|
| PMID |
12459498
|
| MeSH |
Adaptor Proteins, Signal Transducing*
Carrier Proteins / metabolism*
Cell Line
Drosophila Proteins*
Humans
Interleukin-1 / pharmacology*
Membrane Glycoproteins / metabolism
Models, Biological
Mutation
NF-kappa B / metabolism*
Protein Structure, Tertiary
Proteins / chemistry
Proteins / genetics
Proteins / metabolism*
Receptors, Cell Surface / metabolism
Signal Transduction*
TNF Receptor-Associated Factor 6
Toll-Like Receptors
Tumor Necrosis Factor Receptor-Associated Peptides and Proteins
Two-Hybrid System Techniques
|
| IF |
3.057
|
| Times Cited |
36
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| Human and Animal Cells |
|
