| RRC ID |
41765
|
| 著者 |
Hiyoshi M, Ohkubo T, Tsuji K, Hagihara M, Nakasaki H, Mukai M, Makuuchi H, Yamamura M, Tsuda M.
|
| タイトル |
Hepatocyte proliferation factors from neonatal pig liver: purification and characterization.
|
| ジャーナル |
Biofactors
|
| Abstract |
Two factors were found in the condition medium of neonatal pig liver fragments, which were capable of stimulating DNA synthesis in primary hepatocytes. They were named hepatocyte proliferation factor (HPF)-1 and HPF-2 and purified 1,025- and 2,580-fold, respectively. Both HPF-1 and HPF-2 seem to be anionic at pH 8.0 judged from the elution pattern of DEAE (DE52) column chromatography. HPF-1 was recovered as a non-adsorbed fraction in blue Sepharose and heparin Sepharose columns, and had a molecular weight of 26-31 kDa as estimated by gel filtration in high salt condition. Purified HPF-1 stimulated DNA synthesis of primary rat hepatocytes, but suppressed that of HepG2 cells. HPF-2 strongly bound to blue Sepharose and heparin Sepharose columns, and had a molecular weight of 71-90 kDa as estimated by SDS-PAGE under non-reduced condition. Purified HPF-2 stimulated DNA synthesis of primary rat hepatocytes dose dependently but did not suppress that of HepG2 cells. From further biological and chemical characteristics studied in this paper, HPF-1 and HPF-2 may be novel stimulating proteins for hepatocyte proliferation, although the possibility that they are already known growth factors can not be excluded without complete purification and its cloning.
|
| 巻・号 |
16(1-2)
|
| ページ |
1-14
|
| 公開日 |
2002-1-1
|
| DOI |
10.1002/biof.5520160101
|
| PMID |
12515911
|
| MeSH |
Animals
Animals, Newborn*
Carcinoma, Hepatocellular
Cells, Cultured
Chromatography, Gel
DNA / biosynthesis
Drug Stability
Electrophoresis, Polyacrylamide Gel
Growth Substances / chemistry
Growth Substances / isolation & purification*
Growth Substances / pharmacology
Hepatocytes / drug effects
Hepatocytes / metabolism
Hot Temperature
Humans
Hydrogen-Ion Concentration
Liver / chemistry*
Liver Neoplasms
Male
Molecular Weight
Rats
Rats, Wistar
Swine*
Tumor Cells, Cultured
|
| IF |
4.734
|
| 引用数 |
2
|
|
WOS 分野
|
ENDOCRINOLOGY & METABOLISM
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
