論文 - 詳細
| RRC ID | 41769 |
|---|---|
| 著者 | Chang KS, Ohashi K, Onuma M. |
| タイトル | Suppression of transcription activity of the MEQ protein of oncogenic Marek's disease virus serotype 1 (MDV1) by L-MEQ of non-oncogenic MDV1. |
| ジャーナル | J Vet Med Sci |
| Abstract |
Meq is one of the candidate oncogenes in the MDV1 genome. We previously reported a difference in the meq open reading frame (ORF) between oncogenic and non-oncogenic MDV1: L-meq, in which a 180-bp sequence is inserted into the meq ORF, is detected in non-oncogenic MDV1. To study the functions of a gene product of L-meq (L-MEQ), transactivation by L-MEQ was analyzed by dual luciferase assay using a reporter gene under the control of long (-1--873 bp) and short (-1 - -355 bp) meq promoter (LMP and SMP, respectively). LMP showed higher promoter function than SMP. L-MEQ transactivated the expression of the reporter gene, but less than MEQ did. In the presence of SMP or the cytomegalovirus immediate-early promoter, the same or slightly higher transactivation was observed in cells cotransfected with both meq and L-meq than cells transfected only with meq. However, in the presence of LMP, lower transactivation was observed in cells cotransfected with both meq and L-meq than cells transfected only with meq, suggesting that L-MEQ can be a transrepressor. Replication of a vvMDV1 was enhanced in the cells with meq. Interestingly, however, replication of vvMDV1 was suppressed in the cells with L-meq or with both L-meq and meq, compared to untransfected cells. Thus, L-MEQ could suppress replication of vvMDV1 displaying the meq gene in coinfected cells. |
| 巻・号 | 64(12) |
| ページ | 1091-5 |
| 公開日 | 2002-12-1 |
| DOI | 10.1292/jvms.64.1091 |
| PMID | 12520099 |
| MeSH | Animals Antiviral Agents / genetics Antiviral Agents / metabolism Cells, Cultured Gene Expression Regulation, Viral* Herpesvirus 2, Gallid / genetics* Herpesvirus 2, Gallid / metabolism* Humans Mice Oncogene Proteins, Viral / genetics* Oncogene Proteins, Viral / metabolism* Promoter Regions, Genetic / genetics Transcription, Genetic* Transcriptional Activation Tumor Cells, Cultured Virus Replication |
| IF | 1.049 |
| 引用数 | 23 |
| WOS 分野 | VETERINARY SCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 3T3 Swiss Albino(RCB1642) Hep G2 |