RRC ID 41799
Author Ip WK, Wong CK, Lam CW.
Title Tumour necrosis factor-alpha-induced expression of intercellular adhesion molecule-1 on human eosinophilic leukaemia EoL-1 cells is mediated by the activation of nuclear factor-kappaB pathway.
Journal Clin Exp Allergy
Abstract BACKGROUND:Intercellular adhesion molecule-1 (ICAM-1) has been shown to mediate the adhesion and migration of eosinophils to the site of allergic inflammation. However, molecular mechanisms regulating the expression of ICAM-1 in eosinophils are still being elucidated. We investigated the effect of tumour necrosis factor-alpha (TNF-alpha) on ICAM-1 expression of eosinophils.
METHODS:The surface expression of ICAM-1 on a human eosinophilic leukaemic cell line, EoL-1, was assessed by immunocytochemical staining. The phosphorylation of inhibitor kappa B-alpha (IkappaB-alpha) and p38 mitogen-activated protein kinase (MAPK) was detected by Western blot. Nuclear factor kappa-B (NF-kappaB) pathway-related genes were evaluated by the cDNA expression array system, whereas the activity of NF-kappaB was measured by electrophoretic mobility shift assay (EMSA).
RESULTS:TNF-alpha was found to induce the cell surface expression of ICAM-1. A specific proteasome inhibitor N-cbz-Leu-Leu-leucinal (MG-132), but not a p38 MAPK inhibitor (SB 203580), was found to suppress the TNF-alpha-induced expression of ICAM-1 on EoL-1 cells. The gene expressions of ICAM-1, NF-kappaB and IkappaBalpha were up-regulated after the stimulation with TNF-alpha. Further, TNF-alpha was shown to induce IkappaB-alpha phosphorylation and degradation, thereby indicating the activation of NF-kappaB. In EMSA, there was a shifted NF-kappaB band on TNF-alpha-treated cells with or without SB 203580, but no shifted band was observed on MG-132-treated cells.
CONCLUSION:In vitro studies of EoL-1 cells, an eosinophilic leukaemic cell line, confirmed that NF-kappaB plays an important role in the expression of ICAM-1 and recruitment of eosinophils in allergic inflammation.
Volume 33(2)
Pages 241-8
Published 2003-2-1
DOI 10.1046/j.1365-2222.2003.01585.x
PII 1585
PMID 12580918
MeSH Enzyme Inhibitors / pharmacology Eosinophils / metabolism* Gene Expression Regulation / drug effects Humans Hypereosinophilic Syndrome / metabolism I-kappa B Proteins / metabolism Imidazoles / pharmacology Intercellular Adhesion Molecule-1 / metabolism* Leupeptins / pharmacology Mitogen-Activated Protein Kinases / physiology NF-KappaB Inhibitor alpha NF-kappa B / physiology* Phosphorylation / drug effects Pyridines / pharmacology Signal Transduction / genetics Signal Transduction / physiology Tumor Cells, Cultured Tumor Necrosis Factor-alpha / antagonists & inhibitors Tumor Necrosis Factor-alpha / pharmacology* p38 Mitogen-Activated Protein Kinases
IF 4.217
Times Cited 15
Human and Animal Cells EoL-1 cell(RCB0641)