| RRC ID |
41835
|
| Author |
Asano A, Jin HK, Watanabe T.
|
| Title |
Mouse Mx2 gene: organization, mRNA expression and the role of the interferon-response promoter in its regulation.
|
| Journal |
Gene
|
| Abstract |
Mx is an interferon (IFN)-inducible intracellular protein found in various vertebrates that mediates resistance against negative-strand RNA viruses. We have demonstrated previously that feral mouse strains are able to express a functional Mx2 mRNA, while that of the inbred laboratory strains was non-functional because of a single nucleotide insertion in the open reading frame, and under the detectable level. In the present study, we examined the regulation of Mx2 expression in vivo using a congenic mouse carrying Mx1 and Mx2 genes derived from feral strain SPR. Mx2 mRNA was induced strongly in the spleen, ovary and white adipose tissue after the treatment with IFNalpha/beta. Furthermore, we identified the structure of the Mx2 gene. It consists of 14 exons, greatly homologous to the Mx1 gene. The promoter region of Mx2 contained two putative IFN-stimulated response elements (ISREs). We found that the proximal ISRE site positioned between -69 and -55 was essential to IFNalpha/beta-induced transcription by transient transfection assay using reporter gene constructs with mutants of the Mx2 promoter. These results indicate the similarity of the mechanisms of mRNA induction among Mx genes.
|
| Volume |
306
|
| Pages |
105-13
|
| Published |
2003-3-13
|
| DOI |
10.1016/s0378-1119(03)00428-1
|
| PII |
S0378111903004281
|
| PMID |
12657472
|
| MeSH |
3T3 Cells
5' Flanking Region / genetics
Animals
Base Sequence
Cells, Cultured
Cloning, Molecular
Cycloheximide / pharmacology
DNA / chemistry
DNA / genetics
Dactinomycin / pharmacology
Embryo, Mammalian / cytology
Embryo, Mammalian / metabolism
Exons
Female
Fibroblasts / cytology
Fibroblasts / metabolism
GTP-Binding Proteins / genetics*
Gene Expression
Gene Expression Regulation / drug effects
Gene Expression Regulation, Developmental / drug effects
Genes / genetics
Interferons / pharmacology*
Introns
Luciferases / genetics
Luciferases / metabolism
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred Strains
Molecular Sequence Data
Muridae
Mutation
Myxovirus Resistance Proteins
Promoter Regions, Genetic / genetics*
RNA, Messenger / genetics
RNA, Messenger / metabolism*
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / metabolism
Response Elements / genetics
Sequence Analysis, DNA
Sequence Homology, Nucleic Acid
|
| IF |
2.984
|
| Times Cited |
36
|
|
WOS Category
|
GENETICS & HEREDITY
|
| Resource |
| Human and Animal Cells |
|
