論文 - 詳細
| RRC ID | 41855 |
|---|---|
| 著者 | Ueda M, Kokura S, Imamoto E, Naito Y, Handa O, Takagi T, Yoshida N, Yoshikawa T. |
| タイトル | Blocking of NF-kappaB activation enhances the tumor necrosis factor alpha-induced apoptosis of a human gastric cancer cell line. |
| ジャーナル | Cancer Lett |
| Abstract |
The transcription factor NF-kappaB is constitutively activated in many human cancers and induces the expression of multiple genes, including those of anti-apoptotic proteins. This study investigated the mechanism by which human gastric cancer cells (MKN45) are resistant to apoptosis induced by tumor necrosis factor alpha (TNF-alpha). Confluent monolayers of MKN45 cells were either pretreated or not for 60 min with PSI, a peptide aldehyde known to specifically inhibit the chymotrypsin-like activity of 26S proteasome. Cells were subsequently stimulated with recombinant human TNF-alpha, and cell viabilities were determined by the WST-1 assay. Apoptosis was confirmed by fluorescence microscopy after staining with Hoechst 33342, and DNA fragmentation was determined by a DNA fragmentation detection kit. A 24-h incubation with either TNF-alpha or PSI alone did not affect cell viabilities; however, pretreatment with PSI significantly enhanced the level of apoptosis induced by TNF-alpha. Therefore, this study suggests the possibility that blocking of NF-kappaB activity renders gastric cancer cells susceptible to the apoptosis induced by TNF-alpha. |
| 巻・号 | 193(2) |
| ページ | 177-82 |
| 公開日 | 2003-4-25 |
| DOI | 10.1016/s0304-3835(03)00002-8 |
| PII | S0304383503000028 |
| PMID | 12706875 |
| MeSH | Apoptosis* Cell Survival Cysteine Endopeptidases / metabolism DNA Fragmentation Dose-Response Relationship, Drug Humans Microscopy, Fluorescence Multienzyme Complexes / metabolism NF-kappa B / antagonists & inhibitors* NF-kappa B / metabolism* Proteasome Endopeptidase Complex Recombinant Proteins / pharmacology Stomach Neoplasms / metabolism* Time Factors Tumor Cells, Cultured Tumor Necrosis Factor-alpha / metabolism* Ubiquitin / metabolism |
| IF | 7.36 |
| 引用数 | 13 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001) |