論文 - 詳細
| RRC ID | 41872 |
|---|---|
| 著者 | Suda A, Takiguchi Y, Omori S, Miyazawa H, Sugimoto T, Kurosu K, Tatsumi K, Tanabe N, Kuriyama T, Hiroshima K, Kimura H. |
| タイトル | Gene delivery to the lung by means of syngeneic fibroblasts: an experimental model. |
| ジャーナル | Exp Lung Res |
| Abstract |
An experimental model, in which exogenous gene expression in the lung is achieved, has been established. A fibroblast cell line was transfected with the lacZ gene and was administered to syngeneic mice by either intravenous or intratracheal injection. Enzyme-linked immunosorbent assay and histochemical detection of beta-galactosidase revealed efficient gene delivery to the lung by either route. Kinetic studies demonstrated that the expression peaked immediately after the injection, and this high level was maintained for 7 days by intratracheal and for 14 days by intravenous administration. This system may have potential relevance for certain experimental models requiring specific gene delivery to the lung. |
| 巻・号 | 29(4) |
| ページ | 227-41 |
| 公開日 | 2003-6-1 |
| DOI | 10.1080/01902140303781 |
| PII | 1T6DVBP8CRFLPEQU |
| PMID | 12746048 |
| MeSH | Animals Cell Transplantation Female Fibroblasts / cytology Fibroblasts / enzymology* Fibroblasts / transplantation Galactosidases / genetics* Galactosidases / metabolism Gene Transfer Techniques* Gene Transfer, Horizontal* Genetic Vectors Lac Operon / genetics Lung / cytology Lung / enzymology* Mice Mice, Inbred BALB C Models, Animal Specific Pathogen-Free Organisms Transfection |
| IF | 1.702 |
| 引用数 | 2 |
| WOS 分野 | RESPIRATORY SYSTEM |
| リソース情報 | |
| ヒト・動物細胞 | BALB/3T3 clone A31(RCB0005) |