RRC ID 41899
著者 Uda A, Tanabayashi K, Mukai R, Terao K, Yamada A.
タイトル Identification of an amino acid responsible for the CD3 polymorphism in cynomolgus monkeys (Macaca fascicularis).
ジャーナル J Med Primatol
Abstract The FN18 monoclonal antibody (mAb), directed to CD3 molecules, did not react with the lymphocytes of some cynomolgus monkeys (Macaca fascicularis), because of the polymorphism of the CD3epsilon chain. The epitope recognized by the FN18 mAb was successfully expressed on COS7 cells upon transfection of plasmid DNA coding for the CD3epsilon derived from T cells of a FN18 positive cynomolgus monkey. By construction and expression of plasmid DNA encoding the mutant CD3epsilon, the amino acid residue at position 67 was demonstrated to be involved in the formation of an epitope recognizable by the FN18 mAb.
巻・号 32(2)
ページ 105-10
公開日 2003-4-1
DOI 10.1034/j.1600-0684.2003.00012.x
PII 012
PMID 12823633
MeSH Amino Acid Sequence Animals Antibodies, Monoclonal / immunology* Antibody Specificity Base Sequence CD3 Complex / chemistry CD3 Complex / genetics* CD3 Complex / immunology* COS Cells Epitopes / genetics* Epitopes / immunology* Macaca fascicularis / genetics* Macaca fascicularis / immunology Molecular Sequence Data Mutation Polymorphism, Genetic*
IF 0.553
引用数 4
WOS 分野 VETERINARY SCIENCES ZOOLOGY
リソース情報
ヒト・動物細胞 COS-7(RCB0539)