Reference - Detail
| RRC ID | 418 |
|---|---|
| Author | Sassa T, Ueda-Ohba H, Kitamura K, Harada S, Hosono R. |
| Title | Role of Caenorhabditis elegans protein phosphatase type 1, CeGLC-7 beta, in metaphase to anaphase transition during embryonic development. |
| Journal | Exp Cell Res |
| Abstract |
In Caenorhabditis elegans embryogenesis, phosphorylation events are critical to chromosomal changes. To investigate the dephosphorylation of chromosome behavior, we cloned and characterized the cDNA that encodes C. elegans protein phosphatase type 1 (CeGLC-7 beta), which is composed of 333 amino acids. CeGLC-7 beta possesses a highly conserved amino acid sequence with mammalian and Drosophila protein phosphatase 1. Here, we report on the contribution of CeGLC-7 beta to the dephosphorylation of histone H3 at anaphase. At the embryonic stage, CeGLC-7 beta is associated with the nuclear membrane and chromosomes. The deletion of the Ceglc-7 beta gene and a microinjection of double-stranded RNA produce a disorganized embryogenesis. The Ceglc-7 beta gene mutation causes an abnormal accumulation of phosphorylated histone H3 and delays the mitotic process after anaphase. We propose that CeGLC-7 beta is involved in chromosome dynamics including histone H3 dephosphorylation. |
| Volume | 287(2) |
| Pages | 350-60 |
| Published | 2003-7-15 |
| DOI | 10.1016/s0014-4827(03)00157-5 |
| PII | S0014482703001575 |
| PMID | 12837290 |
| MeSH | Amino Acid Sequence Anaphase / genetics Animals Base Sequence Caenorhabditis elegans / embryology Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Conserved Sequence Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Gene Deletion Gene Expression Regulation, Developmental Genes, Helminth* Green Fluorescent Proteins Histones / metabolism Luminescent Proteins / metabolism Metaphase / genetics Molecular Sequence Data Phosphoprotein Phosphatases / genetics Phosphoprotein Phosphatases / metabolism* Phosphorylation Protein Phosphatase 1 RNA, Double-Stranded / metabolism RNA, Small Interfering / metabolism Sequence Homology, Amino Acid |
| IF | 3.383 |
| Times Cited | 6 |
| WOS Category | ONCOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
|
| Total number of mentions | 0 |
| Resource | |
| C.elegans | tm301 |