論文 - 詳細
| RRC ID | 41916 |
|---|---|
| 著者 | Yoshida M, Horiuchi T, Uchinami M, Tabo T, Kimura N, Yokomachi J, Doi K, Nakamura T, Tamagawa K, Tanaka K. |
| タイトル | Intermittent hepatic ischemia-reperfusion minimizes liver metastasis in rats. |
| ジャーナル | J Surg Res |
| Abstract |
BACKGROUND:Surgical stresses, including hepatic ischemia-reperfusion (I/R), promote cancer growth and metastasis. We have reported that continuous hepatic I/R increases liver damage and promoted liver metastasis from colon cancer, whereas intermittent I/R causes less liver damage. We therefore examined whether intermittent I/R could reduce liver metastasis in a rat model. MATERIALS AND METHODS:Adult male Fischer rats was divided between three groups: group A (control), which received laparotomy for 120 min with no liver ischemia; group B (continuous I/R), which received 60 min of 70% partial liver ischemia followed by 60 min of reperfusion; and group C (intermittent I/R), which received 15 min of 70% ischemia and 15 min of reperfusion, repeated four times. Just before closing the abdomen, all animals were inoculated intrasplenically with rat colon adenocarcinoma cells (RCN-H4). Tumor nodules on the liver surface were counted 3 weeks later. In addition, expression of E-selectin mRNA in liver was examined at 1, 3, and 6 h after completing I/R by a reverse transcription-polymerase chain reaction. RESULTS:Continuous I/R (B) greatly promoted liver metastasis in both ischemic and nonischemic liver lobes, whereas intermittent I/R (C) showed significantly fewer metastasis than group B in both lobes. Significantly less E-selectin mRNA was expressed in group C than in group B. CONCLUSIONS:Intermittent I/R limits expression of E-selectin mRNA and liver metastasis. Intermittent hepatic I/R is less stressful than continuous I/R, minimizing liver metastasis by colon cancer cells through avoidance of E-selectin up-regulation. |
| 巻・号 | 111(2) |
| ページ | 255-60 |
| 公開日 | 2003-5-15 |
| DOI | 10.1016/s0022-4804(03)00082-9 |
| PII | S0022480403000829 |
| PMID | 12850471 |
| MeSH | Adenocarcinoma / pathology Adenocarcinoma / secondary Animals Colonic Neoplasms / pathology* E-Selectin / genetics Ischemia* Liver / blood supply* Liver / chemistry Liver / pathology Liver Neoplasms / prevention & control* Liver Neoplasms / secondary* Male Neoplasm Transplantation RNA, Messenger / analysis Rats Reperfusion* Stress, Physiological Surgical Procedures, Operative / adverse effects Tumor Cells, Cultured |
| IF | 1.841 |
| 引用数 | 16 |
| WOS 分野 | SURGERY |
| リソース情報 | |
| ヒト・動物細胞 | RCN-H-4(RCB0512) |