Reference - Detail
| RRC ID | 4195 |
|---|---|
| Author | Zhang L, Ding L, Cheung TH, Dong MQ, Chen J, Sewell AK, Liu X, Yates JR 3rd, Han M. |
| Title | Systematic identification of C. elegans miRISC proteins, miRNAs, and mRNA targets by their interactions with GW182 proteins AIN-1 and AIN-2. |
| Journal | Mol Cell |
| Abstract |
MicroRNAs (miRNAs) regulate gene expression for diverse functions, but only a limited number of mRNA targets have been experimentally identified. We show that GW182 family proteins AIN-1 and AIN-2 act redundantly to regulate the expression of miRNA targets, but not miRNA biogenesis. Immunoprecipitation (IP) and mass spectrometry indicate that AIN-1 and AIN-2 interact only with miRNA-specific Argonaute proteins ALG-1 and ALG-2 and with components of the core translational initiation complex. Known miRNA targets are enriched in AIN-2 complexes, correlating with the expression of corresponding miRNAs. Combining IP with pyrosequencing and microarray analysis of RNAs associated with AIN-1/AIN-2, we identified 106 previously annotated miRNAs plus nine new candidate miRNAs, but nearly no siRNAs, and more than 3500 potential miRNA targets, including nearly all known ones. Our results demonstrate an effective biochemical approach to systematically identify miRNA targets and provide valuable insights regarding the properties of miRNA effector complexes. |
| Volume | 28(4) |
| Pages | 598-613 |
| Published | 2007-11-30 |
| DOI | 10.1016/j.molcel.2007.09.014 |
| PII | S1097-2765(07)00626-0 |
| PMID | 18042455 |
| PMC | PMC2186060 |
| MeSH | 3' Untranslated Regions / metabolism Animals Base Sequence Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology Caenorhabditis elegans / enzymology Caenorhabditis elegans / genetics* Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Carrier Proteins / genetics Carrier Proteins / metabolism* Endoribonucleases / metabolism Eukaryotic Initiation Factor-4E / metabolism Gene Expression Regulation, Developmental MicroRNAs / metabolism* Molecular Sequence Data Mutation / genetics Protein Binding Protein Biosynthesis RNA Caps / metabolism RNA, Helminth / isolation & purification RNA, Helminth / metabolism* RNA, Messenger / genetics RNA, Messenger / metabolism RNA, Small Interfering / metabolism RNA-Induced Silencing Complex / metabolism* Sequence Analysis, RNA Time Factors |
| IF | 15.584 |
| Times Cited | 181 |
| WOS Category | BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 3 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| C.elegans | tm1863 |