| RRC ID |
4195
|
| Author |
Zhang L, Ding L, Cheung TH, Dong MQ, Chen J, Sewell AK, Liu X, Yates JR 3rd, Han M.
|
| Title |
Systematic identification of C. elegans miRISC proteins, miRNAs, and mRNA targets by their interactions with GW182 proteins AIN-1 and AIN-2.
|
| Journal |
Mol Cell
|
| Abstract |
MicroRNAs (miRNAs) regulate gene expression for diverse functions, but only a limited number of mRNA targets have been experimentally identified. We show that GW182 family proteins AIN-1 and AIN-2 act redundantly to regulate the expression of miRNA targets, but not miRNA biogenesis. Immunoprecipitation (IP) and mass spectrometry indicate that AIN-1 and AIN-2 interact only with miRNA-specific Argonaute proteins ALG-1 and ALG-2 and with components of the core translational initiation complex. Known miRNA targets are enriched in AIN-2 complexes, correlating with the expression of corresponding miRNAs. Combining IP with pyrosequencing and microarray analysis of RNAs associated with AIN-1/AIN-2, we identified 106 previously annotated miRNAs plus nine new candidate miRNAs, but nearly no siRNAs, and more than 3500 potential miRNA targets, including nearly all known ones. Our results demonstrate an effective biochemical approach to systematically identify miRNA targets and provide valuable insights regarding the properties of miRNA effector complexes.
|
| Volume |
28(4)
|
| Pages |
598-613
|
| Published |
2007-11-30
|
| DOI |
10.1016/j.molcel.2007.09.014
|
| PII |
S1097-2765(07)00626-0
|
| PMID |
18042455
|
| PMC |
PMC2186060
|
| MeSH |
3' Untranslated Regions / metabolism
Animals
Base Sequence
Caenorhabditis elegans / cytology
Caenorhabditis elegans / embryology
Caenorhabditis elegans / enzymology
Caenorhabditis elegans / genetics*
Caenorhabditis elegans Proteins / genetics
Caenorhabditis elegans Proteins / metabolism*
Carrier Proteins / genetics
Carrier Proteins / metabolism*
Endoribonucleases / metabolism
Eukaryotic Initiation Factor-4E / metabolism
Gene Expression Regulation, Developmental
MicroRNAs / metabolism*
Molecular Sequence Data
Mutation / genetics
Protein Binding
Protein Biosynthesis
RNA Caps / metabolism
RNA, Helminth / isolation & purification
RNA, Helminth / metabolism*
RNA, Messenger / genetics
RNA, Messenger / metabolism
RNA, Small Interfering / metabolism
RNA-Induced Silencing Complex / metabolism*
Sequence Analysis, RNA
Time Factors
|
| IF |
15.584
|
| Times Cited |
181
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
CELL BIOLOGY
|
| Resource |
| C.elegans |
tm1863 |
