| RRC ID |
41967
|
| 著者 |
Nomoto H, Yonezawa T, Itoh K, Ono K, Yamamoto K, Oohashi T, Shiraga F, Ohtsuki H, Ninomiya Y.
|
| タイトル |
Molecular cloning of a novel transmembrane protein MOLT expressed by mature oligodendrocytes.
|
| ジャーナル |
J Biochem
|
| Abstract |
A novel oligodendrocyte (OL)-specific cDNA was isolated from brain capillary endothelial cells and characterized. The cDNA encodes a protein of 1099 amino acids that contains a signal peptide and a transmembrane domain. The protein was expressed in mature OLs in vivo and in vitro cell cultures and was thus designated as mature OL transmembrane protein (MOLT). RT-PCR analysis showed that MOLT mRNA was expressed in brain, lung, pancreas, and testis. A polyclonal antibody raised against a part of the mouse MOLT reacted specifically with multipolar OLs possessing radially oriented processes that penetrated into the gray matter. More cells were detected in the white matter, and these had longitudinally oriented processes. In a rat OL lineage culture system, oligodendrocyte precursor cells did not initially produce MOLT mRNA and protein, but when they begun to differentiate into mature OLs, they started expressing MOLT. Consequently, MOLT may function as OLs become mature and may serve as a cell-surface marker for OL differentiation.
|
| 巻・号 |
134(2)
|
| ページ |
231-8
|
| 公開日 |
2003-8-1
|
| DOI |
10.1093/jb/mvg135
|
| PMID |
12966072
|
| MeSH |
Amino Acid Sequence
Animals
Brain / blood supply
Brain / metabolism
COS Cells
Cell Differentiation / physiology
Cell Lineage
Cells, Cultured
Chlorocebus aethiops
Cloning, Molecular
DNA, Complementary / genetics
DNA, Complementary / isolation & purification
Endothelium, Vascular / chemistry
Endothelium, Vascular / metabolism
Humans
Membrane Proteins / biosynthesis*
Membrane Proteins / genetics*
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Nerve Tissue Proteins / biosynthesis*
Nerve Tissue Proteins / genetics*
Oligodendroglia / cytology
Oligodendroglia / metabolism*
Protein Sorting Signals / physiology
RNA, Messenger / biosynthesis
RNA, Messenger / genetics
Rats
Sequence Homology, Amino Acid
|
| IF |
2.476
|
| 引用数 |
20
|
|
WOS 分野
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
COS-7(RCB0539) |
