論文 - 詳細
| RRC ID | 41971 |
|---|---|
| 著者 | Muneta Y, Kikuma R, Yoshihara K, Mori Y. |
| タイトル | Cloning, expression, and tissue distribution of bovine interleukin-21. |
| ジャーナル | Vet Immunol Immunopathol |
| Abstract |
Bovine interleukin-21 (IL-21) cDNA was cloned and sequenced from bovine peripheral blood lymphocytes (PBLs) stimulated with 10 microg/ml concanavalin A (ConA), 10 microg/ml phytohemagglutinin (PHA), and 50 ng/ml phorbol 12-myristate 13-acetate (PMA) for 48 h. The open reading frame of the bovine IL-21 cDNA is 459 bp in length and encodes 152 amino acids. The predicted amino acid sequence is 78.2 and 58.5% homologous to the human and murine IL-21 amino acid sequences, respectively. Recombinant bovine IL-21 was expressed by a baculovirus expression system. The bovine IL-21 was processed to the mature form in insect cells and secreted to the supernatant confirmed by N-terminal amino acid sequencing. The recombinant bovine mature IL-21 induced the proliferation of human IL-2-dependent cells, ILT-MAT. The mRNA expression for bovine IL-21 was observed in the spleen, but not in the brain, heart, lung, liver, and kidney. The bovine IL-21 identified in this study may provide new methods for the enhancement of innate immunity in cows. |
| 巻・号 | 95(1-2) |
| ページ | 73-80 |
| 公開日 | 2003-9-15 |
| DOI | 10.1016/s0165-2427(03)00106-5 |
| PII | S0165242703001065 |
| PMID | 12969638 |
| MeSH | Amino Acid Sequence Animals Baculoviridae / genetics Base Sequence Cattle / genetics* Cattle / immunology Cell Division / immunology Cells, Cultured Cloning, Molecular DNA, Complementary / genetics Humans Interleukins / genetics* Interleukins / immunology Interleukins / metabolism Interleukins / pharmacology Molecular Sequence Data RNA, Messenger / biosynthesis RNA, Messenger / genetics Recombinant Proteins / biosynthesis Recombinant Proteins / genetics Recombinant Proteins / immunology Recombinant Proteins / pharmacology Reverse Transcriptase Polymerase Chain Reaction Sequence Alignment |
| IF | 1.713 |
| 引用数 | 6 |
| WOS 分野 | VETERINARY SCIENCES IMMUNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | COS-7(RCB0539) |