RRC ID 41972
Author Miyoshi N, Nakamura Y, Ueda Y, Abe M, Ozawa Y, Uchida K, Osawa T.
Title Dietary ginger constituents, galanals A and B, are potent apoptosis inducers in Human T lymphoma Jurkat cells.
Journal Cancer Lett.
Abstract The effects of the constituents isolated from ginger species including curcumin, 6-gingerol and labdane-type diterpene compounds on cell proliferation and the induction of apoptosis in the cultured human T lymphoma Jurkat cells were studied. Among the tested compounds, galanals A and B, isolated from the flower buds of a Japanese ginger, myoga (Zingiber mioga Roscoe), showed the most potent cytotoxic effect. Exposure of Jurkat human T-cell leukemia cells to galanals resulted in the induction of apoptotic cell death characterized by DNA fragmentation and caspase-3 activation. The mitochondrial damage pathway was suggested to be involved in galanal-induced apoptosis because the treatment of cells with galanals induced mitochondrial transmembrane potential (DeltaPsim) alteration and cytochrome c release. The anti-apoptotic Bcl-2 protein was downregulated by the galanal treatment together with enhancement of the Bax expression. In conclusion, the results from this study provide biological evidence that ginger-specific constituents other than curcuminoids are potential anticancer agents.
Volume 199(2)
Pages 113-9
Published 2003-9-25
DOI 10.1016/s0304-3835(03)00381-1
PII S0304383503003811
PMID 12969783
MeSH Apoptosis / drug effects* Blotting, Western Caspase 3 Caspases / metabolism Catechols Curcumin / pharmacology Cytochromes c / metabolism Diet* Diterpenes / pharmacology* Enzyme Activation / drug effects Fatty Alcohols / pharmacology Ginger / chemistry* Humans Jurkat Cells / drug effects Jurkat Cells / metabolism Jurkat Cells / pathology Membrane Potentials / drug effects Mitochondria / drug effects Mutagens / pharmacology Naphthalenes / pharmacology Proto-Oncogene Proteins / metabolism Proto-Oncogene Proteins c-bcl-2 / metabolism Spiro Compounds / pharmacology bcl-2-Associated X Protein
IF 6.491
Times Cited 56
WOS Category ONCOLOGY
Resource
Human and Animal Cells