| RRC ID |
41976
|
| Author |
Koshizuka Y, Yamada T, Hoshi K, Ogasawara T, Chung UI, Kawano H, Nakamura Y, Nakamura K, Ikegawa S, Kawaguchi H.
|
| Title |
Cystatin 10, a novel chondrocyte-specific protein, may promote the last steps of the chondrocyte differentiation pathway.
|
| Journal |
J Biol Chem
|
| Abstract |
This study attempts to characterize cystatin 10 (Cst10), which we recently identified as a novel protein implicated in endochondral ossification. Expression of Cst10 was specific to cartilage, localized in the cytosol of prehypertrophic and hypertrophic chondrocytes of the mouse growth plate. In the mouse chondrogenic cell line ATDC5, Cst10 expression preceded type X collagen expression and increased in synchrony with maturation. When we compared ATDC5 cells transfected with Cst10 cDNA with cells transfected with a mock vector, hypertrophic maturation and mineralization of chondrocytes were promoted by Cst10 gene overexpression in that type X collagen expression was observed earlier, and alizarin red staining was stronger. On the other hand, type II collagen expression and Alcian blue staining, both of which are markers of the early stage of chondrocyte differentiation, were similar in both cells. Overexpression of the Cst10 gene also caused fragmentation of nuclei, the appearance of annexin V, a change in the mitochondrial membrane potential, and activation of caspases. These results strongly suggest that Cst10 may play an important role in the last steps of the chondrocyte differentiation pathway as an inducer of maturation, followed by apoptosis of chondrocytes.
|
| Volume |
278(48)
|
| Pages |
48259-66
|
| Published |
2003-11-28
|
| DOI |
10.1074/jbc.M211639200
|
| PII |
S0021-9258(20)75862-X
|
| PMID |
13679380
|
| MeSH |
Alcian Blue / metabolism
Amino Acid Sequence
Animals
Annexin A5 / biosynthesis
Anthraquinones / pharmacology
Apoptosis
Blotting, Western
Cartilage / metabolism
Cell Differentiation
Cell Line
Cell Membrane / metabolism
Cell Nucleus / metabolism
Cells, Cultured
Chondrocytes / metabolism*
Chromosomes / metabolism
Collagen / metabolism
Coloring Agents / pharmacology
Cystatins / chemistry
Cystatins / physiology*
DNA, Complementary / metabolism
Genetic Vectors
Immunoblotting
Immunohistochemistry
In Situ Hybridization, Fluorescence
Membrane Potentials
Mice
Mice, Transgenic
Microscopy, Fluorescence
Molecular Sequence Data
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Time Factors
Transfection
|
| IF |
4.238
|
| Times Cited |
16
|
|
WOS Category
|
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
ATDC5(RCB0565) |
