論文 - 詳細
| RRC ID | 42007 |
|---|---|
| 著者 | Sasaki E, Okamoto Y, Yoshida K, Okamura H, Shimizu K, Nasu F, Morimoto H, Haneji T. |
| タイトル | Transblot and cytochemical identification of avidin-interacting proteins in mitochondria of cultured cells. |
| ジャーナル | Histochem Cell Biol |
| Abstract |
Cell lysates prepared from 3T3-L1 cells were analyzed by western blotting using the avidin-biotin complex system and anti-Bax antibody. The antibody interacted with bands of proteins with estimated molecular weights of 120, 74, 72, and 25 kDa. However, only the 25-kDa band was detected with the anti-Bax antibody when the direct immunoblotting method was used. Peroxidase-conjugated avidin interacted with the 120-, 74-, and 72-kDa bands. This interaction was not limited to 3T3-L1 cells, because peroxidase-avidin also interacted with these three proteins in MC3T3-E1, YROS, Saos-2, MG63, SCCKN, and SCCTF cells although the staining intensity was different in each cell type. Avidin-peroxidase also interacted with these three proteins in the mitochondria-containing fractions prepared from 3T3-L1 cells. FITC-streptavidin was also localized in mitochondria in the cultured cells. The localization of avidin/streptavidin-interacting proteins in mitochondria was confirmed by using double staining with FITC-streptavidin and Mito-tracker. |
| 巻・号 | 120(4) |
| ページ | 327-33 |
| 公開日 | 2003-10-1 |
| DOI | 10.1007/s00418-003-0572-x |
| PMID | 14574588 |
| MeSH | 3T3-L1 Cells / chemistry 3T3-L1 Cells / ultrastructure Animals Blotting, Western Cells, Cultured Electrophoresis, Polyacrylamide Gel Indicators and Reagents Mice Mitochondria / chemistry* Mitochondrial Proteins / analysis* Proto-Oncogene Proteins / analysis Proto-Oncogene Proteins c-bcl-2* Streptavidin* bcl-2-Associated X Protein |
| IF | 3.418 |
| 引用数 | 4 |
| WOS 分野 | MICROSCOPY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | SCCKN(RCB0441) SCCTF(RCB0442) |