論文 - 詳細
| RRC ID | 42018 |
|---|---|
| 著者 | Aoshima Y, Hasegawa Y, Hasegawa S, Nagasaka A, Kimura T, Hashimoto S, Torii Y, Tsukagoshi N. |
| タイトル | Isolation of GnafC, a polysaccharide constituent of Gnaphalium affine, and synergistic effects of GnafC and ascorbate on the phenotypic expression of osteoblastic MC3T3-E1 cells. |
| ジャーナル | Biosci Biotechnol Biochem |
| Abstract |
After screening extensively factors in plant extracts that increase alkaline phosphatase activity, an osteoblastic differentiation marker protein in mouse calvarial osteoblast MC3T3-E1 cells, GnafC derived from Gnaphalium affine, was found to significantly enhance the alkaline phosphatase (ALPase) activity in a synergistic manner with ascorbate. GnafC was a polysaccharaide with an approximate molecular mass of 10,000 and comprised mannose, xylose, arabinose, galactose and glucose in a molar ratio of 1:2:4.3:2.5:2.7. Expression of the osteoblastic differentiation marker genes was examined by semiquantitative RT-PCR with RNAs prepared from cells at different developmental stages. With ascorbate in the culture, GnafC enhanced the expression of the ALPase and MMP13 genes from the early stage of differentiation, leading to maturation of the collagenous extracellular matrix (ECM), a prerequisite for mineralization. |
| 巻・号 | 67(10) |
| ページ | 2068-74 |
| 公開日 | 2003-10-1 |
| DOI | 10.1271/bbb.67.2068 |
| PMID | 14586092 |
| MeSH | Alkaline Phosphatase / biosynthesis Alkaline Phosphatase / genetics Animals Ascorbic Acid / pharmacology* Cell Differentiation / drug effects Cell Differentiation / genetics Cell Line Collagenases / biosynthesis Collagenases / genetics Drug Synergism Gene Expression Profiling Gene Expression Regulation / drug effects Matrix Metalloproteinase 13 Mice Osteoblasts / cytology Osteoblasts / drug effects* Phenotype Plant Extracts / isolation & purification Plant Extracts / pharmacology Polysaccharides / isolation & purification* Polysaccharides / pharmacology* RNA, Messenger / analysis |
| IF | 1.516 |
| 引用数 | 6 |
| WOS 分野 | CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY BIOTECHNOLOGY & APPLIED MICROBIOLOGY BIOCHEMISTRY & MOLECULAR BIOLOGY |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |