論文 - 詳細
| RRC ID | 42038 |
|---|---|
| 著者 | Yanai A, Hirata Y, Mitsuno Y, Maeda S, Shibata W, Akanuma M, Yoshida H, Kawabe T, Omata M. |
| タイトル | Helicobacter pylori induces antiapoptosis through buclear factor-kappaB activation. |
| ジャーナル | J Infect Dis |
| Abstract |
Although Helicobacter pylori is classified as a definite carcinogen, the mechanism underlying gastric carcinogenesis is not yet clear. We previously have shown that H. pylori activates an antiapoptotic gene, the cellular inhibitor of apoptosis protein 2 (c-IAP2), the underlying mechanism of which was investigated in the present study. cDNA array and real-time PCR analyses indicated that H. pylori showed a stimulatory effect on the expression of c-IAP2. Isogenic mutant strains with impaired cag pathogenicity island (cagPAI) expression showed weaker induction. Analyses that used the in situ terminal deoxynucleotide transferase-mediated dUTP nick end-labeling method indicated suppression of antiapoptosis by the antisense c-IAP2 oligonucleotide. Reporter assays with deletion and mutation constructs for the c-IAP2 promoter showed that nuclear factor-kappaB (NF-kappaB) binding sites are indispensable for transactivation. Super-repressor IkappaBalpha or NF-kappaB inhibitor reduced c-IAP2 transactivation by H. pylori, and exogenous expression of c-IAP2 inhibited apoptosis seen with H. pylori. In conclusion, H. pylori induces antiapoptosis through c-IAP2 transactivation following cagPAI-dependent NF-kappaB activation. The interaction of these stimuli may play a role in gastric carcinogenesis. |
| 巻・号 | 188(11) |
| ページ | 1741-51 |
| 公開日 | 2003-12-1 |
| DOI | 10.1086/379629 |
| PII | JID30721 |
| PMID | 14639546 |
| MeSH | Apoptosis* Cell Line, Tumor Helicobacter pylori / pathogenicity* Humans Inhibitor of Apoptosis Proteins NF-kappa B / physiology* Promoter Regions, Genetic Proteins / genetics Stomach Neoplasms / pathology Transcriptional Activation |
| IF | 5.022 |
| 引用数 | 41 |
| WOS 分野 | INFECTIOUS DISEASES IMMUNOLOGY MICROBIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MKN45(RCB1001) |