RRC ID |
42045
|
著者 |
Hirano M, Yamamoto A, Yoshimura N, Tokunaga T, Motohashi T, Ishizaki K, Yoshida H, Okazaki K, Yamazaki H, Hayashi S, Kunisada T.
|
タイトル |
Generation of structures formed by lens and retinal cells differentiating from embryonic stem cells.
|
ジャーナル |
Dev Dyn
|
Abstract |
Embryonic stem cells have the potential to give rise to all cell lineages when introduced into the early embryo. They also give rise to a limited number of different cell types in vitro in specialized culture systems. In this study, we established a culture system in which a structure consisting of lens, neural retina, and pigmented retina was efficiently induced from embryonic stem cells. Refractile cell masses containing lens and neural retina were surrounded by retinal pigment epithelium layers and, thus, designated as eye-like structures. Developmental processes required for eye development appear to proceed in this culture system, because the formation of the eye-like structures depended on the expression of Pax6, a key transcription factor for eye development. The present culture system opens up the possibility of examining early stages of eye development and also of producing cells for use in cellular therapy for various diseases of the eye.
|
巻・号 |
228(4)
|
ページ |
664-71
|
公開日 |
2003-12-1
|
DOI |
10.1002/dvdy.10425
|
PMID |
14648843
|
MeSH |
Animals
Cell Differentiation
Cell Line
Cells, Cultured
Embryo, Mammalian / cytology*
Eye Proteins
Gene Expression Regulation, Developmental*
Green Fluorescent Proteins
Homeodomain Proteins / physiology
Immunohistochemistry
Lens, Crystalline / embryology*
Luminescent Proteins / metabolism
Mice
Microscopy, Fluorescence
Neurons / metabolism
PAX6 Transcription Factor
Paired Box Transcription Factors
Repressor Proteins
Retina / embryology*
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells / cytology*
Time Factors
|
IF |
3.275
|
引用数 |
67
|
WOS 分野
|
ANATOMY & MORPHOLOGY
DEVELOPMENTAL BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
anti-αA-crystallin(RCB1303)
anti-αB-crystallin(RCB1304) |