論文 - 詳細
| RRC ID | 42061 |
|---|---|
| 著者 | Yamamoto M, Kuroiwa A. |
| タイトル | Hoxa-11 and Hoxa-13 are involved in repression of MyoD during limb muscle development. |
| ジャーナル | Dev Growth Differ |
| Abstract |
Under the influence of the limb mesenchyme, Hoxa-11 is expressed in migrating and proliferating premyoblasts in the limb field and Hoxa-13 is induced in subdomains of congregated limb muscle masses. To evaluate the roles of Hoxa-11 and Hoxa-13 in myogenesis of the limb, we performed electroporation in ovo to force expression of these Hox genes in limb muscle precursors. In the presence of ectopic Hoxa-11, expression of MyoD was blocked transiently. In C2C12 myoblasts, transfection of Hoxa-11 also repressed the expression of endogenous MyoD. Forced expression of Hoxa-13 resulted in more pronounced repression of MyoD in both limb and C2C12 myoblasts. In contrast, targeted disruption of Hoxa-13 gave rise to enhanced expression of MyoD in the flexor carpi radialis muscle, a forearm muscle that normally expressed Hoxa-13. These results suggest that Hoxa-11 and Hoxa-13 are involved in the negative regulation of MyoD expression in limb muscle precursors. |
| 巻・号 | 45(5-6) |
| ページ | 485-98 |
| 公開日 | 2003-1-1 |
| DOI | 10.1111/j.1440-169x.2003.00715.x |
| PMID | 14706073 |
| MeSH | Animals Body Patterning Cell Line Chick Embryo Chickens Codon DNA, Complementary / metabolism Electroporation Extremities / embryology* Gene Expression Regulation, Developmental Homeodomain Proteins / physiology* Immunohistochemistry In Situ Hybridization Mice Muscles / embryology* MyoD Protein / metabolism* Protein Structure, Tertiary Time Factors Transfection |
| IF | 1.723 |
| 引用数 | 31 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY CELL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | F1000Research |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | C2C12(RCB0987) |