論文 - 詳細
| RRC ID | 42065 |
|---|---|
| 著者 | Ishii T, Fujishiro M, Masuda M, Nakajima J, Teramoto S, Ouchi Y, Matsuse T. |
| タイトル | Depletion of glutathione S-transferase P1 induces apoptosis in human lung fibroblasts. |
| ジャーナル | Exp Lung Res |
| Abstract |
Glutathione S-transferase P1 (GSTP1) is one of the xenobiotic-metabolizing and antioxidant enzymes, identified in the peripheral lungs. Recently, the authors reported the association between GSTP1 gene polymorphism and susceptibility to chronic obstructive pulmonary disease (COPD), and protective effect of GSTP1 against cigarette smoke in human lung fibroblasts in vitro. In this study, the authors investigated that depletion of GSTP1 by itself could induce cell death, including apoptosis, in human lung fibroblast-derived HFL-1 cells. The level of apoptosis and necrosis was increased significantly with GSTP1 antisense vector transfection. It was also observed that the transfection efficiency and the expression level of the vector were weaker in the transfectant of the antisense vector than in those of the sense and control vectors, which is also thought to indicate that inhibition of GSTP1 expression by the antisense vector alone affects cellular viability. However, there was no difference among these transfectants neither on glutathione (GSH) level nor on c-Jun NH2-terminal kinase (JNK) activation. Therefore, the authors report here that underexpression of GSTP1 appeared to induce apoptosis on lung fibroblasts, which suggests that GSTP1 may have protective effects against apoptosis in the airway cells, though the mechanism of this apoptotic pathway is still to be elucidated. |
| 巻・号 | 29(7) |
| ページ | 523-36 |
| 公開日 | 2003-1-1 |
| DOI | 10.1080/01902140303777 |
| PMID | 14710442 |
| MeSH | Apoptosis* Cell Count Cells, Cultured Fibroblasts / enzymology* Fibroblasts / pathology* Flow Cytometry Genetic Vectors Glutathione / analysis Glutathione / biosynthesis Glutathione S-Transferase pi Glutathione Transferase / deficiency* Glutathione Transferase / genetics Humans Isoenzymes / deficiency* Isoenzymes / genetics JNK Mitogen-Activated Protein Kinases Lung / cytology* Mitogen-Activated Protein Kinases / biosynthesis Necrosis Oligonucleotides, Antisense / genetics Transfection |
| IF | 1.702 |
| 引用数 | 22 |
| WOS 分野 | RESPIRATORY SYSTEM |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HFL-I(RCB0521) |