論文 - 詳細
| RRC ID | 42086 |
|---|---|
| 著者 | Itoh M, Shimazu A, Hirata I, Yoshida Y, Shintani H, Okazaki M. |
| タイトル | Characterization of CO3Ap-collagen sponges using X-ray high-resolution microtomography. |
| ジャーナル | Biomaterials |
| Abstract |
For reconstruction and regeneration of hard tissues, scaffold biomaterials with large size pores and high porosity are important, in addition to their roles as supporting frames. To develop a new biodegradable scaffold biomaterial, CO3Ap, which has crystallinity and a chemical composition similar to bone, was synthesized at pH 7.4 and 60 degrees C. Then, the CO3Ap was mixed with a neutralized collagen gel and the CO3Ap-collagen mixtures with different kinds of CO3Ap contents and porosity were lyophilized into sponges. Scanning electron micrography (SEM) observation of CO3Ap-collagen sponges showed favorable pores for cell invasion. Approximately 50-300 microm size pores appeared to continue through the bulk. Higher magnification of the sponge showed a better adhesion between CO3Ap crystals and collagen. X-ray high-resolution microtomography revealed a clear image of the 3D structure of the sponges. The porosity of 0, 70 and 90%(w/w) CO3Ap-collagen sponges was 79.2 +/- 2.8%, 72.6 +/- 2.4% and 48.9 +/- 6.1%, respectively. The 70%(w/w) CO3Ap-collagen sponge appeared to be the most favorable biomaterial from the viewpoint of natural bone properties. Mouse osteoblast MC3T3-E1 cells were cultured in alphaMEM with 10% FCS for 2 weeks. Hematoxylin-eosin staining confirmed osteoblast cells invaded well into the CO3Ap-collagen sponge. These sponges are expected to be used as hard tissue scaffold biomaterials for therapeutic uses. |
| 巻・号 | 25(13) |
| ページ | 2577-83 |
| 公開日 | 2004-6-1 |
| DOI | 10.1016/j.biomaterials.2003.09.071 |
| PII | S0142961203007634 |
| PMID | 14751743 |
| MeSH | 3T3 Cells Animals Biocompatible Materials* Cattle Collagen / chemistry* Mice Microscopy, Electron, Scanning Tomography, X-Ray / methods* X-Ray Diffraction |
| IF | 10.317 |
| 引用数 | 38 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 17 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 3.0 |
| リソース情報 | |
| ヒト・動物細胞 | MC3T3-E1(RCB1126) |