RRC ID 42091
著者 Binnerts ME, Wen X, Canté-Barrett K, Bright J, Chen HT, Asundi V, Sattari P, Tang T, Boyle B, Funk W, Rupp F.
タイトル Human Crossveinless-2 is a novel inhibitor of bone morphogenetic proteins.
ジャーナル Biochem Biophys Res Commun
Abstract Drosophila Crossveinless-2 (dCV-2) is required for local activation of Mad phosphorylation in the fruit fly wing and has been postulated to be a positive regulator of BMP-mediated signaling. In contrast, the presence of 5 Chordin-like cysteine-rich domains in the CV-2 protein suggests that CV-2 belongs to a family of well-established inhibitors of BMP function that includes Chordin and Sog [Development 127 (2000) 3947]. We have identified a human homolog of Drosophila CV-2 (hCV-2). Here we show that purified recombinant hCV-2 protein inhibits BMP-2 and BMP-4 dependent osteogenic differentiation of W-20-17 cells, as well as BMP dependent chondrogenic differentiation of ATDC5 cells. Interestingly, hCV-2 messenger RNA is expressed at high levels in human primary chondrocytes, whereas expression in primary human osteoblasts is low. These results suggest that hCV-2 may regulate BMP responsiveness of osteoblasts and chondrocytes in vivo. Taken together we have shown that contrary to the function predicted from the fruit fly, Crossveinless-2 is a novel inhibitor of BMP function.
巻・号 315(2)
ページ 272-80
公開日 2004-3-5
DOI 10.1016/j.bbrc.2004.01.048
PII S0006291X04000890
PMID 14766204
MeSH Alkaline Phosphatase / metabolism Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Protein 4 Bone Morphogenetic Proteins / antagonists & inhibitors* Bone Morphogenetic Proteins / metabolism Cell Differentiation Cell Division Cell Line Cells, Cultured Cysteine / chemistry DNA, Complementary / metabolism Dose-Response Relationship, Drug Drosophila Drosophila Proteins / metabolism Drosophila Proteins / physiology* Glycoproteins / chemistry Humans Intercellular Signaling Peptides and Proteins / chemistry Mice Osteoblasts / metabolism Phenotype Phosphorylation Protein Structure, Tertiary RNA, Messenger / metabolism Recombinant Proteins / chemistry Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Tissue Distribution Transforming Growth Factor beta*
IF 2.985
引用数 60
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY
リソース情報
ヒト・動物細胞