論文 - 詳細
| RRC ID | 42091 |
|---|---|
| 著者 | Binnerts ME, Wen X, Canté-Barrett K, Bright J, Chen HT, Asundi V, Sattari P, Tang T, Boyle B, Funk W, Rupp F. |
| タイトル | Human Crossveinless-2 is a novel inhibitor of bone morphogenetic proteins. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Drosophila Crossveinless-2 (dCV-2) is required for local activation of Mad phosphorylation in the fruit fly wing and has been postulated to be a positive regulator of BMP-mediated signaling. In contrast, the presence of 5 Chordin-like cysteine-rich domains in the CV-2 protein suggests that CV-2 belongs to a family of well-established inhibitors of BMP function that includes Chordin and Sog [Development 127 (2000) 3947]. We have identified a human homolog of Drosophila CV-2 (hCV-2). Here we show that purified recombinant hCV-2 protein inhibits BMP-2 and BMP-4 dependent osteogenic differentiation of W-20-17 cells, as well as BMP dependent chondrogenic differentiation of ATDC5 cells. Interestingly, hCV-2 messenger RNA is expressed at high levels in human primary chondrocytes, whereas expression in primary human osteoblasts is low. These results suggest that hCV-2 may regulate BMP responsiveness of osteoblasts and chondrocytes in vivo. Taken together we have shown that contrary to the function predicted from the fruit fly, Crossveinless-2 is a novel inhibitor of BMP function. |
| 巻・号 | 315(2) |
| ページ | 272-80 |
| 公開日 | 2004-3-5 |
| DOI | 10.1016/j.bbrc.2004.01.048 |
| PII | S0006291X04000890 |
| PMID | 14766204 |
| MeSH | Alkaline Phosphatase / metabolism Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Protein 4 Bone Morphogenetic Proteins / antagonists & inhibitors* Bone Morphogenetic Proteins / metabolism Cell Differentiation Cell Division Cell Line Cells, Cultured Cysteine / chemistry DNA, Complementary / metabolism Dose-Response Relationship, Drug Drosophila Drosophila Proteins / metabolism Drosophila Proteins / physiology* Glycoproteins / chemistry Humans Intercellular Signaling Peptides and Proteins / chemistry Mice Osteoblasts / metabolism Phenotype Phosphorylation Protein Structure, Tertiary RNA, Messenger / metabolism Recombinant Proteins / chemistry Reverse Transcriptase Polymerase Chain Reaction Signal Transduction Tissue Distribution Transforming Growth Factor beta* |
| IF | 2.985 |
| 引用数 | 60 |
| WOS 分野 | BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |