RRC ID |
42091
|
著者 |
Binnerts ME, Wen X, Canté-Barrett K, Bright J, Chen HT, Asundi V, Sattari P, Tang T, Boyle B, Funk W, Rupp F.
|
タイトル |
Human Crossveinless-2 is a novel inhibitor of bone morphogenetic proteins.
|
ジャーナル |
Biochem Biophys Res Commun
|
Abstract |
Drosophila Crossveinless-2 (dCV-2) is required for local activation of Mad phosphorylation in the fruit fly wing and has been postulated to be a positive regulator of BMP-mediated signaling. In contrast, the presence of 5 Chordin-like cysteine-rich domains in the CV-2 protein suggests that CV-2 belongs to a family of well-established inhibitors of BMP function that includes Chordin and Sog [Development 127 (2000) 3947]. We have identified a human homolog of Drosophila CV-2 (hCV-2). Here we show that purified recombinant hCV-2 protein inhibits BMP-2 and BMP-4 dependent osteogenic differentiation of W-20-17 cells, as well as BMP dependent chondrogenic differentiation of ATDC5 cells. Interestingly, hCV-2 messenger RNA is expressed at high levels in human primary chondrocytes, whereas expression in primary human osteoblasts is low. These results suggest that hCV-2 may regulate BMP responsiveness of osteoblasts and chondrocytes in vivo. Taken together we have shown that contrary to the function predicted from the fruit fly, Crossveinless-2 is a novel inhibitor of BMP function.
|
巻・号 |
315(2)
|
ページ |
272-80
|
公開日 |
2004-3-5
|
DOI |
10.1016/j.bbrc.2004.01.048
|
PII |
S0006291X04000890
|
PMID |
14766204
|
MeSH |
Alkaline Phosphatase / metabolism
Animals
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins / antagonists & inhibitors*
Bone Morphogenetic Proteins / metabolism
Cell Differentiation
Cell Division
Cell Line
Cells, Cultured
Cysteine / chemistry
DNA, Complementary / metabolism
Dose-Response Relationship, Drug
Drosophila
Drosophila Proteins / metabolism
Drosophila Proteins / physiology*
Glycoproteins / chemistry
Humans
Intercellular Signaling Peptides and Proteins / chemistry
Mice
Osteoblasts / metabolism
Phenotype
Phosphorylation
Protein Structure, Tertiary
RNA, Messenger / metabolism
Recombinant Proteins / chemistry
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction
Tissue Distribution
Transforming Growth Factor beta*
|
IF |
2.985
|
引用数 |
60
|
WOS 分野
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
リソース情報 |
ヒト・動物細胞 |
|