Reference - Detail
RRC ID | 42096 |
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Author | Takahashi M, Kobayashi Y. |
Title | Cytokine production in association with phagocytosis of apoptotic cells by immature dendritic cells. |
Journal | Cell Immunol |
Abstract |
Immature dendritic cells (iDCs) can ingest apoptotic cells, which do not lead to maturation of the iDCs. In this paper we examine the phagocytosis of apoptotic cells by iDCs in the absence of stimuli for the maturation of iDCs and the subsequent cytokine production. Phagocytosis was observed by confocal microscopy, and it increased as apoptosis proceeded. The coculturing of iDCs with apoptotic cells did not induce the maturation of iDCs even after the subsequent LPS treatment, as assessed as to the expression of MHC class II, CD80, CD86, and CD40. Moreover, IL-6 and IL-12p40 among the cytokines examined were specifically up-regulated by the coculturing at the mRNA and protein levels. The coculturing decreased the expression of MHC class II on iDCs and allogenic T cell proliferation induced by iDCs. Although anti-IL-6 antibodies only partially reversed the effect of coculturing with apoptotic cells, exogenous IL-6 decreased significantly the expression of MHC class II on iDCs and allogenic T cell proliferation induced by iDCs, raising the possibility that IL-6 may be partly involved in maintaining the immature status of iDCs in an autocrine manner. |
Volume | 226(2) |
Pages | 105-15 |
Published | 2003-12-1 |
DOI | 10.1016/j.cellimm.2003.11.008 |
PII | S0008874903002843 |
PMID | 14962498 |
MeSH | Animals Apoptosis / immunology* CD36 Antigens / immunology Cells, Cultured Coculture Techniques Dendritic Cells / drug effects Dendritic Cells / immunology* Down-Regulation Flow Cytometry Histocompatibility Antigens Class II / drug effects Histocompatibility Antigens Class II / immunology Interleukin-12 / biosynthesis Interleukin-12 / immunology Interleukin-12 Subunit p40 Interleukin-6 / biosynthesis Interleukin-6 / immunology* Interleukin-6 / pharmacology Lymphocyte Culture Test, Mixed Male Mice Microscopy, Confocal Phagocytosis / immunology* Protein Subunits / biosynthesis Protein Subunits / immunology Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes / drug effects T-Lymphocytes / immunology |
IF | 4.078 |
Times Cited | 27 |
WOS Category | IMMUNOLOGY CELL BIOLOGY |
Resource | |
Human and Animal Cells |