論文 - 詳細
| RRC ID | 42100 |
|---|---|
| 著者 | Rawat SS, Eaton J, Gallo SA, Martin TD, Ablan S, Ratnayake S, Viard M, KewalRamani VN, Wang JM, Blumenthal R, Puri A. |
| タイトル | Functional expression of CD4, CXCR4, and CCR5 in glycosphingolipid-deficient mouse melanoma GM95 cells and susceptibility to HIV-1 envelope glycoprotein-triggered membrane fusion. |
| ジャーナル | Virology |
| Abstract |
We had previously reported that glycosphingolipids (GSL) support human immunodeficiency virus type 1 (HIV-1) entry. In this study, we further examined this issue by expressing HIV-1 receptors in GSL-deficient GM95 cells. GM95 cells expressing low levels of CD4 and CXCR4 or CCR5 did not support HIV-1 Env-mediated fusion. However, higher expression of these receptors rendered GM95 cells highly susceptible to fusion with cells expressing appropriate HIV-1 envelope glycoproteins (HIV-1 Envs). The GM95 cells exhibited a different fusion phenotype when compared with GSL(+) NIH3T3 cells bearing similar receptor levels. Fusion of GM95 targets expressing higher levels of CD4 and coreceptors occurred at 25 degrees C and was sensitive to cholesterol depletion or disruption of the cytoskeleton. In contrast, the fusion threshold of NIH3T3CD4X4/R5 targets was at >/=28 degrees C as previously reported and was insensitive to cholesterol depletion or cytoskeletal network disruption. On the basis of these observations, we propose that target membrane GSLs support HIV-1 Env-mediated fusion at low density of receptors by stabilizing receptor pools in natural targets. |
| 巻・号 | 318(1) |
| ページ | 55-65 |
| 公開日 | 2004-1-5 |
| DOI | 10.1016/j.virol.2003.08.042 |
| PII | S0042682203006639 |
| PMID | 14972535 |
| MeSH | Animals CD4 Antigens / metabolism* Gene Products, env / pharmacology* Glycosphingolipids / deficiency* Glycosphingolipids / metabolism HIV-1 / pathogenicity HIV-2 / pathogenicity HeLa Cells Humans Melanoma Membrane Fusion / drug effects* Mice NIH 3T3 Cells Receptors, CCR5 / metabolism* Receptors, CXCR4 / metabolism* Tumor Cells, Cultured |
| IF | 2.819 |
| 引用数 | 17 |
| WOS 分野 | VIROLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | GM95(RCB1026) |