RRC ID 42125
著者 Woo JT, Nakagawa H, Notoya M, Yonezawa T, Udagawa N, Lee IS, Ohnishi M, Hagiwara H, Nagai K.
タイトル Quercetin suppresses bone resorption by inhibiting the differentiation and activation of osteoclasts.
ジャーナル Biol Pharm Bull
Abstract Although quercetin has suppressed bone resorption in several animal studies, its target cells and the mechanism of its action related to bone resorption has not been fully elucidated. We investigated the effect of quercetin on the differentiation and activation of osteoclasts. We used cocultures of mouse spleen cells and ST2 cells, and cultures of osteoclast progenitor cells [M-CSF-dependent (MD) cells from mouse bone marrow and murine monocytic RAW 264 (RAW) cells]. Quercetin dose-dependently inhibited osteoclast-like (OCL) cell formation at 2-5 microM concentration in both the coculture and MD cell culture. Quercetin inhibited the increase of tartrate-resistant acid phosphatase (TRAP) activity of mononuclear preosteoclasts (pOCs) induced by receptor activator of nuclear factor-kappaB (NF-kappaB) ligand (RANKL) in both MD and RAW cell cultures. Quercetin reversely induced the disruption of actin rings in OCLs. Quercetin also suppressed both pit formation induced by osteoclasts on dentine slices and PTH-stimulated (45)Ca release in mouse long bone cultures. These results suggest that osteoclast progenitors as well as mature osteoclasts, are quercetin's target cells in relation to bone resorption, and that quercetin's suppressive effect on bone resorption results from both its inhibitory effect on the differentiation of osteoclast progenitor cells into pOCs and from its disruptive effect on actin rings in mature osteoclasts.
巻・号 27(4)
ページ 504-9
公開日 2004-4-1
DOI 10.1248/bpb.27.504
PMID 15056855
MeSH Acid Phosphatase / metabolism Actins / metabolism Actins / ultrastructure Animals Bone Marrow Cells / cytology Bone Marrow Cells / drug effects Bone Resorption / pathology Bone Resorption / prevention & control* Carrier Proteins / pharmacology Cell Differentiation / drug effects Cell Survival / drug effects Cells, Cultured Coculture Techniques Isoenzymes / metabolism Male Membrane Glycoproteins / pharmacology Mice Monocytes / cytology Monocytes / drug effects Osteoclasts / cytology Osteoclasts / drug effects* Quercetin / pharmacology* RANK Ligand Receptor Activator of Nuclear Factor-kappa B Tartrate-Resistant Acid Phosphatase
IF 1.863
引用数 79
WOS 分野 PHARMACOLOGY & PHARMACY
リソース情報
ヒト・動物細胞 ST2(RCB0224)