| RRC ID |
42130
|
| Author |
Dirgahayu P, Fukumoto S, Tademoto S, Kina Y, Hirai K.
|
| Title |
Excretory/secretory products from plerocercoids of Spirometra erinaceieuropaei suppress interleukin-1beta gene expression in murine macrophages.
|
| Journal |
Int J Parasitol
|
| Abstract |
The present study shows that ES products from plerocercoids of Spirometra erinaceieuropaei suppressed interleukin-1beta mRNA expression in lipopolysaccharide-stimulated RAW 264.7 macrophages in the absence or presence of a cyclic AMP analogue, dibutyryl cyclic AMP. Investigation using the inhibitors of mitogen-activated protein kinase (MAPK) pathways revealed that extracellular signal-regulated protein kinase 1/2 and p38 mitogen-activated protein kinase pathways are crucial for full induction of interleukin-1beta mRNA expression. ES products additionally suppressed interleukin-1beta mRNA expression in the cells treated with p38 mitogen-activated protein kinase inhibitor (SB203580) or extracellular signal-regulated protein kinase 1/2 inhibitor (PD98059). Western blot analysis showed that dibutyryl cyclic AMP enhanced lipopolysaccharide-induced phosphorylation of extracellular signal-regulated protein kinase 1/2, p38 mitogen-activated protein kinase and cyclic AMP responsive element binding protein (CREB) and, in turn, we demonstrated that ES products reduced the lipopolysaccharide and dibutyryl cyclic AMP-induced phosphorylation of extracellular signal-regulated protein kinase 1/2 and p38 mitogen-activated protein kinase, but not cyclic AMP responsive element binding protein. These data demonstrate that ES products from the plerocercoids of S. erinaceieuropaei may evade induction of interleukin-1beta mRNA by inhibiting extracellular signal-regulated protein kinase 1/2 and p38 mitogen-activated protein kinase pathways in lipopolysaccharide and/or dibutyryl cyclic AMP-stimulated macrophages.
|
| Volume |
34(5)
|
| Pages |
577-84
|
| Published |
2004-4-1
|
| DOI |
10.1016/j.ijpara.2003.12.006
|
| PII |
S0020751904000037
|
| PMID |
15064122
|
| MeSH |
Animals
Blotting, Northern / methods
Blotting, Western / methods
Bucladesine / genetics
Cell Line
Cyclic AMP Response Element-Binding Protein / genetics
Gene Expression Regulation / genetics
Interleukin-1 / genetics*
Lipopolysaccharides
MAP Kinase Signaling System / genetics
Macrophages / physiology*
Mice
Mitogen-Activated Protein Kinase 3
Mitogen-Activated Protein Kinases / genetics
Phosphorylation
RNA, Helminth / genetics
RNA, Messenger / genetics
Signal Transduction / genetics
Spirometra / genetics
Spirometra / physiology*
|
| IF |
3.53
|
| Times Cited |
9
|
|
WOS Category
|
PARASITOLOGY
|
| Resource |
| Human and Animal Cells |
RAW 264(RCB0535) |
