論文 - 詳細
| RRC ID | 4214 |
|---|---|
| 著者 | Budirahardja Y, Gönczy P. |
| タイトル | PLK-1 asymmetry contributes to asynchronous cell division of C. elegans embryos. |
| ジャーナル | Development |
| Abstract |
Acquisition of lineage-specific cell cycle duration is an important feature of metazoan development. In Caenorhabditis elegans, differences in cell cycle duration are already apparent in two-cell stage embryos, when the larger anterior blastomere AB divides before the smaller posterior blastomere P1. This time difference is under the control of anterior-posterior (A-P) polarity cues set by the PAR proteins. The mechanisms by which these cues regulate the cell cycle machinery differentially in AB and P1 are incompletely understood. Previous work established that retardation of P1 cell division is due in part to preferential activation of an ATL-1/CHK-1 dependent checkpoint in P1, but how the remaining time difference is controlled is not known. Here, we establish that differential timing relies also on a mechanism that promotes mitosis onset preferentially in AB. The polo-like kinase PLK-1, a positive regulator of mitotic entry, is distributed in an asymmetric manner in two-cell stage embryos, with more protein present in AB than in P1. We find that PLK-1 asymmetry is regulated by A-P polarity cues through preferential protein retention in the embryo anterior. Importantly, mild inactivation of plk-1 by RNAi delays entry into mitosis in P1, but not in AB, in a manner that is independent of ATL-1/CHK-1. Together, our findings support a model in which differential timing of mitotic entry in C. elegans embryos relies on two complementary mechanisms: ATL-1/CHK-1-dependent preferential retardation in P1 and PLK-1-dependent preferential promotion in AB, which together couple polarity cues and cell cycle progression during early development. |
| 巻・号 | 135(7) |
| ページ | 1303-13 |
| 公開日 | 2008-4-1 |
| DOI | 10.1242/dev.019075 |
| PII | dev.019075 |
| PMID | 18305005 |
| MeSH | Animals Animals, Genetically Modified Caenorhabditis elegans / cytology Caenorhabditis elegans / embryology* Caenorhabditis elegans / physiology* Cell Cycle Proteins / metabolism* Cell Division* Cell Polarity / genetics Embryo, Nonmammalian / cytology Embryo, Nonmammalian / metabolism Mitosis / physiology Models, Biological Polo-Like Kinase 1 Protein Serine-Threonine Kinases / metabolism* Proto-Oncogene Proteins / metabolism* |
| IF | 5.611 |
| 引用数 | 48 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm853 |