論文 - 詳細
| RRC ID | 42155 |
|---|---|
| 著者 | Hirai F, Motoori S, Kakinuma S, Tomita K, Indo HP, Kato H, Yamaguchi T, Yen HC, St Clair DK, Nagano T, Ozawa T, Saisho H, Majima HJ. |
| タイトル | Mitochondrial signal lacking manganese superoxide dismutase failed to prevent cell death by reoxygenation following hypoxia in a human pancreatic cancer cell line, KP4. |
| ジャーナル | Antioxid Redox Signal |
| Abstract |
One of the major characteristics of tumor is the presence of a hypoxic cell population, which is caused by abnormal distribution of blood vessels. Manganese superoxide dismutase (MnSOD) is a nuclear-encoded mitochondrial enzyme, which scavenges superoxide generated from the electron-transport chain in mitochondria. We examined whether MnSOD protects against hypoxia/reoxygenation (H/R)-induced oxidative stress using a human pancreas carcinoma-originated cell line, KP4. We also examined whether MnSOD is necessarily present in mitochondria to have a function. Normal human MnSOD and MnSOD without a mitochondrial targeting signal were transfected to KP4 cells, and reactive oxygen species, nitric oxide, lipid peroxidation, and apoptosis were examined as a function of time in air following 1 day of hypoxia as a H/R model. Our results showed H/R caused no increase in nitric oxide, but resulted in increases in reactive oxygen species, 4-hydroxy-2-nonenal protein adducts, and apoptosis. Authentic MnSOD protected against these processes and cell death, but MnSOD lacking a mitochondrial targeting signal could not. These results suggest that only when MnSOD is located in mitochondria is it efficient in protecting against cellular injuries by H/R, and they also indicate that mitochondria are primary sites of H/R-induced cellular oxidative injuries. |
| 巻・号 | 6(3) |
| ページ | 523-35 |
| 公開日 | 2004-6-1 |
| DOI | 10.1089/152308604773934288 |
| PMID | 15130279 |
| MeSH | Aldehydes / chemistry Apoptosis Cell Death Cell Line, Tumor DNA Adducts DNA Primers / chemistry DNA, Complementary / metabolism Humans Hypoxia Lipid Peroxidation Microscopy, Fluorescence Mitochondria / metabolism Mitochondria / pathology* Nitric Oxide / metabolism Oxidative Stress Oxygen / metabolism RNA, Messenger / metabolism Reactive Oxygen Species / metabolism Reverse Transcriptase Polymerase Chain Reaction Signal Transduction* Superoxide Dismutase / metabolism Superoxide Dismutase / physiology* Time Factors Transfection |
| IF | 7.04 |
| 引用数 | 21 |
| WOS 分野 | ENDOCRINOLOGY & METABOLISM BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | KP4(RCB1005) |