| RRC ID |
42163
|
| 著者 |
Nakatsu Y, Yamada K, Ueda J, Onogi A, Ables GP, Nishibori M, Hata H, Takada A, Sawai K, Tanabe Y, Morita M, Daikohara M, Watanabe T.
|
| タイトル |
Genetic polymorphisms and antiviral activity in the bovine MX1 gene.
|
| ジャーナル |
Anim Genet
|
| Abstract |
Bovine MX1 cDNAs consisting of 2280 bp from 11 animals of five breeds and from a cultured cell line were sequenced and compared with previously reported data. Ten nucleotide substitutions were synonymous mutations, and a single nucleotide substitution at 458 resulted in an amino acid exchange of Ile (ATT) and Met (ATG). A 13-bp deletion-insertion mutation was also found in the 3'-UTR. Based on the nucleotide substitutions found in this study, bovine MX1 cDNA was classified into 11 genotypes. A phylogenetic tree of the 11 genotypes suggested that the genotypes observed in Brahman were a great genetic distance from other genotypes. An 18-bp deletion-insertion variation at position 171 was found to be the result of alternative splicing. The 18-bp deletion-insertion is located at the boundary between exon 3 and intron 3. Permanently transfected 3T3 cell lines expressing bovine MX1 mRNA were established to analyse the antiviral potential against VSVDeltaG*-G infection. Transfected cell clones expressing bovine MX1 mRNA showed a significantly smaller number of cells infected with VSVDeltaG*-G compared with the control cells. These results indicate that the bovine MX1 protein has potent antiviral activity.
|
| 巻・号 |
35(3)
|
| ページ |
182-7
|
| 公開日 |
2004-6-1
|
| DOI |
10.1111/j.1365-2052.2004.01125.x
|
| PII |
AGE1125
|
| PMID |
15147388
|
| MeSH |
3T3 Cells
Alternative Splicing
Animals
Antiviral Agents / genetics*
Base Sequence
Cattle / genetics*
Cluster Analysis
DNA Primers
DNA, Complementary / genetics
GTP-Binding Proteins / classification
GTP-Binding Proteins / genetics*
Mice
Molecular Sequence Data
Mutation, Missense / genetics
Myxovirus Resistance Proteins
Phylogeny*
Polymorphism, Genetic*
Reverse Transcriptase Polymerase Chain Reaction
Sequence Analysis, DNA
Transfection
Vesicular stomatitis Indiana virus / genetics*
|
| IF |
2.841
|
| 引用数 |
15
|
|
WOS 分野
|
AGRICULTURE, DAIRY & ANIMAL SCIENCE
GENETICS & HEREDITY
|
| リソース情報 |
| ヒト・動物細胞 |
3T3 fibroblast cells from a BALB/c mouse |
