RRC ID 42175
著者 Hayashi T, Mizuno N, Ueda Y, Okamoto M, Kondoh H.
タイトル FGF2 triggers iris-derived lens regeneration in newt eye.
ジャーナル Mech Dev
Abstract Lens regeneration in newts occurs exclusively from the dorsal aspect of the iris pigment epithelium. Although the phenomenon has been a paradigm of experimental tissue regeneration, little is understood about how it is initiated and restricted to the dorsal iris. Here we show among various growth factors injected in an intact eye, a single injection of FGF2 specifically caused morphological changes of the iris characteristic of lens regeneration, induced expression of transcription factor genes Pax6, Sox2 and MafB, as well as endogenous Fgf2 in both dorsal and ventral halves, and provoked second lens development only from the dorsal iris. FGF2 protein accumulated in the iris tissue after the lens was removed, and injection of a soluble form of FGF receptor titrating FGF2 inhibited all reactions observed after the lens removal or after administration of FGF2. These results indicate that FGF2 and/or related molecules trigger lens regeneration from the dorsal iris in the newt. The observations also indicate that the absence of lens regeneration from the ventral iris is due to a block in a later phase of lens developmental pathway.
巻・号 121(6)
ページ 519-26
公開日 2004-6-1
DOI 10.1016/j.mod.2004.04.010
PII S0925477304000917
PMID 15172683
MeSH Animals Bromodeoxyuridine / pharmacology Cell Proliferation Coloring Agents / pharmacology DNA-Binding Proteins / metabolism Eye Proteins Fibroblast Growth Factor 2 / physiology* Gene Expression Regulation, Developmental* HMGB Proteins High Mobility Group Proteins / metabolism Homeodomain Proteins / metabolism Immunohistochemistry Iris / metabolism* Lens, Crystalline / growth & development Lens, Crystalline / physiology* MafB Transcription Factor Nuclear Proteins / metabolism Oncogene Proteins / metabolism PAX6 Transcription Factor Paired Box Transcription Factors Phosphorylation RNA / metabolism Receptors, Fibroblast Growth Factor / metabolism Regeneration* Repressor Proteins Reverse Transcriptase Polymerase Chain Reaction SOXB1 Transcription Factors Salamandridae Time Factors Tissue Distribution Transcription Factors / metabolism Tumor Suppressor Proteins Xenopus Proteins / metabolism
IF 2.126
引用数 44
WOS 分野 DEVELOPMENTAL BIOLOGY
リソース情報
ヒト・動物細胞