論文 - 詳細
| RRC ID | 42196 |
|---|---|
| 著者 | Tanaka K, Asanuma M, Ogawa N. |
| タイトル | Molecular basis of anti-apoptotic effect of immunophilin ligands on hydrogen peroxide-induced apoptosis in human glioma cells. |
| ジャーナル | Neurochem Res |
| Abstract |
To clarify the molecular basis of the cytoprotective properties of immunophilin ligands (IPLs), the anti-apoptotic effects of IPLs were determined in human glioma U251 cells. GPI1046 and V10367, non-immunosuppressive IPLs (NI-IPLs), as well as FK506, an immunosuppressive IPL (I-IPL), had cytoprotective effects against hydrogen peroxide (H20O)-induced apoptotic cell death in U251 cells. H2O2 increased both the ratio of bax/bcl-2 and the p53 mRNA expression. However, pre-treatment with FK506 and V10367 significantly prevented any increase in this ratio or p53 mRNA expression. GPI1046 also reduced the ratio of bax/bcl-2 to the normal level. In addition, H2O2 significantly increased activities of all three caspases, caspase-3, caspase-8, and caspase-9, in comparison with non-H2O2 controls. However, FK506 prevented the increase of these caspase activities. On the other hand, it is well-known that glutathione (GSH) and neurotrophic factor (NTF) is related to the induction of apoptosis in neuronal cells. In U251 cells, FK506, GPI1046 and V10367 had GSH-activating and NTF-activating effects. Thus, the immunosuppressive effect is not essential for the cytoprotective properties of IPLs, and IPLs have multiple beneficial properties such as the anti-apoptotic effect, GSH-activating effect, and NTF-activating effect, although the anti-apoptotic effect of NI-IPLs is independent of the regulation of apoptotic activators such as caspase-3. |
| 巻・号 | 29(8) |
| ページ | 1529-36 |
| 公開日 | 2004-8-1 |
| DOI | 10.1023/b:nere.0000029565.92587.25 |
| PMID | 15260130 |
| MeSH | Apoptosis / drug effects* Brain Neoplasms Brain-Derived Neurotrophic Factor / metabolism Cell Line, Tumor Cell Survival / drug effects* Glial Cell Line-Derived Neurotrophic Factor Glioma Glutathione / metabolism Humans Hydrogen Peroxide / pharmacology* Immunophilins / physiology* Ligands Nerve Growth Factors / metabolism Pyridines / pharmacology* Pyrrolidines / pharmacology* Tacrolimus / pharmacology* |
| IF | 3.038 |
| 引用数 | 20 |
| WOS 分野 | NEUROSCIENCES BIOCHEMISTRY & MOLECULAR BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 14 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | U251(RCB0461) |