| RRC ID |
42202
|
| 著者 |
Ishii T, Fujishiro M, Masuda M, Teramoto S, Matsuse T.
|
| タイトル |
A methylated oligonucleotide induced methylation of GSTP1 promoter and suppressed its expression in A549 lung adenocarcinoma cells.
|
| ジャーナル |
Cancer Lett
|
| Abstract |
Glutathione S-transferase P1 (GSTP1) belongs to xenobiotic enzymes, and is supposed to contribute to chemoresistance. Though it was reported that GSTP1 gene is suppressed by cytosine-guanine (CpG) island methylation of its promoter, this promoter is not strongly methylated and GSTP1 protein is highly expressed in lung cancer. We intended to induce methylation of GSTP1 CpG island by using a methylated sense oligonucleotide complementary to this region. When we transduced the methylated oligonucleotides to A549 lung adenocarcinoma cells, methylation of the GSTP1 promoter and reduction of GSTP1 expression was induced, cell viability was reduced; however, chemoresistance against cisplatin has not clearly changed.
|
| 巻・号 |
212(2)
|
| ページ |
211-23
|
| 公開日 |
2004-8-30
|
| DOI |
10.1016/j.canlet.2004.03.001
|
| PII |
S0304383504001764
|
| PMID |
15279901
|
| MeSH |
Apoptosis
Base Sequence
Cell Line, Tumor
Cell Survival
Cisplatin / pharmacology
CpG Islands
Cytosine / chemistry
DNA Methylation*
DNA Primers / chemistry
Drug Resistance, Neoplasm
Flow Cytometry
Genetic Techniques*
Glutathione Transferase / genetics*
Guanine / chemistry
Humans
Immunoblotting
Models, Genetic
Molecular Sequence Data
Oligonucleotides / genetics*
Polymerase Chain Reaction
Promoter Regions, Genetic*
RNA, Small Interfering
Time Factors
Transfection
|
| IF |
7.36
|
| 引用数 |
19
|
|
WOS 分野
|
ONCOLOGY
|
| リソース情報 |
| ヒト・動物細胞 |
|
