論文 - 詳細
| RRC ID | 42202 |
|---|---|
| 著者 | Ishii T, Fujishiro M, Masuda M, Teramoto S, Matsuse T. |
| タイトル | A methylated oligonucleotide induced methylation of GSTP1 promoter and suppressed its expression in A549 lung adenocarcinoma cells. |
| ジャーナル | Cancer Lett |
| Abstract |
Glutathione S-transferase P1 (GSTP1) belongs to xenobiotic enzymes, and is supposed to contribute to chemoresistance. Though it was reported that GSTP1 gene is suppressed by cytosine-guanine (CpG) island methylation of its promoter, this promoter is not strongly methylated and GSTP1 protein is highly expressed in lung cancer. We intended to induce methylation of GSTP1 CpG island by using a methylated sense oligonucleotide complementary to this region. When we transduced the methylated oligonucleotides to A549 lung adenocarcinoma cells, methylation of the GSTP1 promoter and reduction of GSTP1 expression was induced, cell viability was reduced; however, chemoresistance against cisplatin has not clearly changed. |
| 巻・号 | 212(2) |
| ページ | 211-23 |
| 公開日 | 2004-8-30 |
| DOI | 10.1016/j.canlet.2004.03.001 |
| PII | S0304383504001764 |
| PMID | 15279901 |
| MeSH | Apoptosis Base Sequence Cell Line, Tumor Cell Survival Cisplatin / pharmacology CpG Islands Cytosine / chemistry DNA Methylation* DNA Primers / chemistry Drug Resistance, Neoplasm Flow Cytometry Genetic Techniques* Glutathione Transferase / genetics* Guanine / chemistry Humans Immunoblotting Models, Genetic Molecular Sequence Data Oligonucleotides / genetics* Polymerase Chain Reaction Promoter Regions, Genetic* RNA, Small Interfering Time Factors Transfection |
| IF | 7.36 |
| 引用数 | 19 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | |