論文 - 詳細
| RRC ID | 4222 |
|---|---|
| 著者 | Dittman JS, Kaplan JM. |
| タイトル | Behavioral impact of neurotransmitter-activated G-protein-coupled receptors: muscarinic and GABAB receptors regulate Caenorhabditis elegans locomotion. |
| ジャーナル | J Neurosci |
| Abstract |
Neurotransmitter released from presynaptic terminals activates both ligand-gated ion channels (ionotropic receptors) and a variety of G-protein-coupled receptors (GPCRs). These neurotransmitter receptors are expressed on both presynaptic and postsynaptic cells. Thus, each neurotransmitter acts on multiple receptor classes, generating a large repertoire of physiological responses. The impact of many ionotropic receptors on neuronal activity and behavior has been clearly elucidated; however, much less is known about how neurotransmitter-gated GPCRs regulate neurons and circuits. In Caenorhabditis elegans, both acetylcholine (ACh) and GABA are released in the nerve cord and mediate fast neuromuscular excitation and inhibition during locomotion. Here we identify a muscarinic receptor (GAR-2) and the GABA(B) receptor dimer (GBB-1/2) that detect synaptically released ACh and GABA, respectively. Both GAR-2 and GBB-1/2 inhibited cholinergic motor neurons when ACh and GABA levels were enhanced. Loss of either GPCR resulted in movement defects, suggesting that these receptors are activated during locomotion. When the negative feedback provided by GAR-2 was replaced with positive feedback, animals became highly sensitive to ACh levels and locomotion was severely impaired. Thus, conserved GPCRs act in the nematode motor circuit to provide negative feedback and to regulate locomotory behaviors that underlie navigation. |
| 巻・号 | 28(28) |
| ページ | 7104-12 |
| 公開日 | 2008-7-9 |
| DOI | 10.1523/JNEUROSCI.0378-08.2008 |
| PII | 28/28/7104 |
| PMID | 18614679 |
| PMC | PMC2679701 |
| MeSH | Acetylcholine / metabolism* Aldicarb / pharmacology Animals Animals, Genetically Modified Antinematodal Agents / pharmacology Behavior, Animal / physiology Caenorhabditis elegans / physiology* Caenorhabditis elegans Proteins / genetics Cholinesterase Inhibitors / pharmacology Gene Expression Regulation / drug effects Gene Expression Regulation / physiology Levamisole / pharmacology Locomotion / physiology* Luminescent Proteins / genetics Luminescent Proteins / metabolism Mutation Neurons / drug effects Receptors, Cholinergic / physiology* Receptors, GABA-B / physiology* gamma-Aminobutyric Acid / metabolism* |
| IF | 5.674 |
| 引用数 | 49 |
| WOS 分野 | NEUROSCIENCES |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 線虫 | tm1406 tm1165 |