| RRC ID |
42242
|
| 著者 |
Konno T, Hasuda H, Ishihara K, Ito Y.
|
| タイトル |
Photo-immobilization of a phospholipid polymer for surface modification.
|
| ジャーナル |
Biomaterials
|
| Abstract |
A photo-reactive polymer having a phospholipid polar group was prepared, and the polymer was photo-immobilized on polymeric surfaces, where its interactions with biocomponents were investigated. By using a photo-immobilization method, the polymer was used for surface modification of polyethylene and polypropylene, polymers whose surfaces were not treated in our previous development of the phosphorylcholine-derived polymer. The photo-reactive polymer was synthesized by a coupling reaction involving copolymer consisting of 2-methacryloyloxyethyl phosphorylcholine and methacrylic acid with 4-azidoaniline. When the polymer was unpattern immobilized on the surface, X-ray photo-electron spectroscopic analysis and static contact angle measurements were performed. It was shown that the surface was covered with phospholipid polar groups. Micropattern immobilization was carried out using a micropatterned photo-mask. Measurements using atomic force microscopy showed that the swelled micropatterned polymer was five times as thick as the dried one. Protein adsorption and platelet adhesion were reduced on the polymer-immobilized regions. Mammalian cells did not adhere, and formed aggregates on the immobilized regions. In conclusion, the photo-reactive phospholipid polymer was covalently immobilized on the conventional polymer surfaces and it tended to reduce interactions with proteins and cells.
|
| 巻・号 |
26(12)
|
| ページ |
1381-8
|
| 公開日 |
2005-4-1
|
| DOI |
10.1016/j.biomaterials.2004.04.047
|
| PII |
S0142961204004466
|
| PMID |
15482825
|
| MeSH |
Adsorption / radiation effects
Animals
Cell Adhesion / physiology*
Cells, Cultured
Coated Materials, Biocompatible / chemistry
Humans
Immunoglobulins / chemistry
Light
Materials Testing
Mice
Monocytes / cytology
Monocytes / physiology
Phospholipids / chemistry*
Phospholipids / radiation effects
Photochemistry / methods*
Platelet Adhesiveness / physiology*
Polyethylene / chemistry*
Polyethylene / radiation effects
Polypropylenes / chemistry*
Polypropylenes / radiation effects
Protein Binding
Serum Albumin, Bovine / chemistry
Surface Properties
Tissue Engineering / methods
|
| IF |
10.317
|
| 引用数 |
60
|
|
WOS 分野
|
ENGINEERING, BIOMEDICAL
MATERIALS SCIENCE, BIOMATERIALS
|
| リソース情報 |
| ヒト・動物細胞 |
RAW 264(RCB0535) |
