| RRC ID |
4229
|
| 著者 |
Gissendanner CR, Kelley K, Nguyen TQ, Hoener MC, Sluder AE, Maina CV.
|
| タイトル |
The Caenorhabditis elegans NR4A nuclear receptor is required for spermatheca morphogenesis.
|
| ジャーナル |
Dev Biol
|
| Abstract |
The gene nhr-6 encodes the Caenorhabditis elegans ortholog of the NR4A nuclear receptor. We determined the biological functions of NHR-6 through the isolation and characterization of a deletion allele of nhr-6, lg6001. We demonstrate that nhr-6 has an essential role in the development of the C. elegans somatic gonad. Specifically, nhr-6 is required for the development of the hermaphrodite spermatheca, a somatic gonad organ that serves as the site of sperm storage and oocyte fertilization. Using a variety of spermatheca cell markers, we have determined that loss of nhr-6 function causes severe morphological defects in the spermatheca and associated spermathecal valves. This appears to be due to specific requirements for nhr-6 in regulating cell proliferation and cell differentiation during development of these structures. The improper development of these structures in nhr-6(lg6001) mutants leads to defects in ovulation and significantly reduced fecundity of C. elegans hermaphrodites. The phenotypes of nhr-6(lg6001) mutants are consistent with a role for nhr-6 in organogenesis, similar to the functions of its mammalian homologs.
|
| 巻・号 |
313(2)
|
| ページ |
767-86
|
| 公開日 |
2008-1-15
|
| DOI |
10.1016/j.ydbio.2007.11.014
|
| PII |
S0012-1606(07)01544-8
|
| PMID |
18096150
|
| PMC |
PMC3845373
|
| MeSH |
Alleles
Animals
Animals, Genetically Modified
Caenorhabditis elegans / embryology*
Caenorhabditis elegans / genetics
Caenorhabditis elegans Proteins / physiology
Cell Differentiation
Cell Proliferation
Crosses, Genetic
Disorders of Sex Development
Embryo, Nonmammalian
Female
Fertilization / physiology
Gene Deletion
Genes, Helminth*
Genes, Reporter
Gonads / cytology
Green Fluorescent Proteins / metabolism
Immunohistochemistry
Male
Mutation
Oocytes / physiology
Organ Size
Organogenesis*
Ovulation
Protein Isoforms / genetics
Protein Isoforms / metabolism
RNA Interference
RNA, Messenger / genetics
RNA, Messenger / metabolism
Receptors, Cytoplasmic and Nuclear / genetics
Receptors, Cytoplasmic and Nuclear / physiology*
Spermatozoa / physiology
|
| IF |
2.896
|
| 引用数 |
21
|
|
WOS 分野
|
DEVELOPMENTAL BIOLOGY
|
| リソース情報 |
| 線虫 |
tm870 |
