論文 - 詳細
| RRC ID | 4229 |
|---|---|
| 著者 | Gissendanner CR, Kelley K, Nguyen TQ, Hoener MC, Sluder AE, Maina CV. |
| タイトル | The Caenorhabditis elegans NR4A nuclear receptor is required for spermatheca morphogenesis. |
| ジャーナル | Dev Biol |
| Abstract |
The gene nhr-6 encodes the Caenorhabditis elegans ortholog of the NR4A nuclear receptor. We determined the biological functions of NHR-6 through the isolation and characterization of a deletion allele of nhr-6, lg6001. We demonstrate that nhr-6 has an essential role in the development of the C. elegans somatic gonad. Specifically, nhr-6 is required for the development of the hermaphrodite spermatheca, a somatic gonad organ that serves as the site of sperm storage and oocyte fertilization. Using a variety of spermatheca cell markers, we have determined that loss of nhr-6 function causes severe morphological defects in the spermatheca and associated spermathecal valves. This appears to be due to specific requirements for nhr-6 in regulating cell proliferation and cell differentiation during development of these structures. The improper development of these structures in nhr-6(lg6001) mutants leads to defects in ovulation and significantly reduced fecundity of C. elegans hermaphrodites. The phenotypes of nhr-6(lg6001) mutants are consistent with a role for nhr-6 in organogenesis, similar to the functions of its mammalian homologs. |
| 巻・号 | 313(2) |
| ページ | 767-86 |
| 公開日 | 2008-1-15 |
| DOI | 10.1016/j.ydbio.2007.11.014 |
| PII | S0012-1606(07)01544-8 |
| PMID | 18096150 |
| PMC | PMC3845373 |
| MeSH | Alleles Animals Animals, Genetically Modified Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / physiology Cell Differentiation Cell Proliferation Crosses, Genetic Disorders of Sex Development Embryo, Nonmammalian Female Fertilization / physiology Gene Deletion Genes, Helminth* Genes, Reporter Gonads / cytology Green Fluorescent Proteins / metabolism Immunohistochemistry Male Mutation Oocytes / physiology Organ Size Organogenesis* Ovulation Protein Isoforms / genetics Protein Isoforms / metabolism RNA Interference RNA, Messenger / genetics RNA, Messenger / metabolism Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / physiology* Spermatozoa / physiology |
| IF | 2.896 |
| 引用数 | 21 |
| WOS 分野 | DEVELOPMENTAL BIOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| 線虫 | tm870 |