RRC ID 4229
Author Gissendanner CR, Kelley K, Nguyen TQ, Hoener MC, Sluder AE, Maina CV.
Title The Caenorhabditis elegans NR4A nuclear receptor is required for spermatheca morphogenesis.
Journal Dev Biol
Abstract The gene nhr-6 encodes the Caenorhabditis elegans ortholog of the NR4A nuclear receptor. We determined the biological functions of NHR-6 through the isolation and characterization of a deletion allele of nhr-6, lg6001. We demonstrate that nhr-6 has an essential role in the development of the C. elegans somatic gonad. Specifically, nhr-6 is required for the development of the hermaphrodite spermatheca, a somatic gonad organ that serves as the site of sperm storage and oocyte fertilization. Using a variety of spermatheca cell markers, we have determined that loss of nhr-6 function causes severe morphological defects in the spermatheca and associated spermathecal valves. This appears to be due to specific requirements for nhr-6 in regulating cell proliferation and cell differentiation during development of these structures. The improper development of these structures in nhr-6(lg6001) mutants leads to defects in ovulation and significantly reduced fecundity of C. elegans hermaphrodites. The phenotypes of nhr-6(lg6001) mutants are consistent with a role for nhr-6 in organogenesis, similar to the functions of its mammalian homologs.
Volume 313(2)
Pages 767-86
Published 2008-1-15
DOI 10.1016/j.ydbio.2007.11.014
PII S0012-1606(07)01544-8
PMID 18096150
PMC PMC3845373
MeSH Alleles Animals Animals, Genetically Modified Caenorhabditis elegans / embryology* Caenorhabditis elegans / genetics Caenorhabditis elegans Proteins / physiology Cell Differentiation Cell Proliferation Crosses, Genetic Disorders of Sex Development Embryo, Nonmammalian Female Fertilization / physiology Gene Deletion Genes, Helminth* Genes, Reporter Gonads / cytology Green Fluorescent Proteins / metabolism Immunohistochemistry Male Mutation Oocytes / physiology Organ Size Organogenesis* Ovulation Protein Isoforms / genetics Protein Isoforms / metabolism RNA Interference RNA, Messenger / genetics RNA, Messenger / metabolism Receptors, Cytoplasmic and Nuclear / genetics Receptors, Cytoplasmic and Nuclear / physiology* Spermatozoa / physiology
IF 2.896
Times Cited 21
C.elegans tm870