| RRC ID |
42314
|
| 著者 |
Iwamoto Y, Matsumoto Y, Ueoka R.
|
| タイトル |
Induction of apoptosis of human lung carcinoma cells by hybrid liposomes containing polyoxyethylenedodecyl ether.
|
| ジャーナル |
Int J Pharm
|
| Abstract |
Hybrid liposomes can be prepared by simply ultrasonicating a mixture of vesicular and micellar molecules in aqueous solution. A clear solution of hybrid liposomes composed of 90 mol% dimyristoylphosphatidylcholine (DMPC) and 10 mol% polyoxyethylene(23)dodecyl ether (C12(EO)23) having a hydrodynamic diameter of 100-120 nm was obtained. Highly inhibitory effects of hybrid liposomes of 90 mol% DMPC/10 mol% C12(EO)23 on the growth of human lung carcinoma (RERF-LC-OK and A549) cells without any drugs were obtained. Induction of apoptosis by hybrid liposomes in RERF-LC-OK and A549 cells was verified on the basis of fluorescence microscopy, agarose gel electrophoresis of DNA and flow cytometry. We elucidated the pathway of apoptosis induced by hybrid liposomes as follows: (a) accumulation of hybrid liposomes in tumor cell membrane was revealed using microphysiometer. (b) Reduction of mitochodrial membrane potential and activation of caspase-9, -3 and -8 were obtained, indicating that apoptotic signal by hybrid liposomes should pass through mitochondria and these caspases. It is worthy to note that such a novel mechanism of apoptosis induced by hybrid liposomes without any drugs was performed for the first time in human lung carcinoma cells.
|
| 巻・号 |
292(1-2)
|
| ページ |
231-9
|
| 公開日 |
2005-3-23
|
| DOI |
10.1016/j.ijpharm.2004.11.034
|
| PII |
S0378-5173(04)00705-7
|
| PMID |
15725570
|
| MeSH |
Biosensing Techniques / methods
Caspases / drug effects
Caspases / metabolism
Cell Line, Tumor*
Cell Membrane / drug effects
Cell Membrane / metabolism
Cell Proliferation / drug effects
DNA Fragmentation / drug effects*
DNA Fragmentation / physiology
Dimyristoylphosphatidylcholine / analysis
Dimyristoylphosphatidylcholine / pharmacokinetics
Drug Screening Assays, Antitumor
Humans
Liposomes / analysis*
Liposomes / chemical synthesis
Liposomes / pharmacokinetics
Lung Neoplasms / pathology
Microscopy, Fluorescence / methods
Nanotechnology
Polidocanol
Polyethylene Glycols / analysis*
Polyethylene Glycols / pharmacokinetics*
Signal Transduction / drug effects
Signal Transduction / physiology
Technology, Pharmaceutical / methods
Technology, Pharmaceutical / trends
|
| IF |
4.845
|
| 引用数 |
34
|
|
WOS 分野
|
PHARMACOLOGY & PHARMACY
|
| リソース情報 |
| ヒト・動物細胞 |
A549(RCB0098) |
