RRC ID 4232
Author Gravato-Nobre MJ, Hodgkin J.
Title The acyltransferase gene bus-1 exhibits conserved and specific expression in nematode rectal cells and reveals pathogen-induced cell swelling.
Journal Dev. Dyn.
Abstract Susceptibility to the rectal pathogen Microbacterium nematophilum provides a means of examining hindgut differentiation in C. elegans. Mutants of bus-1 are resistant to infection with this pathogen. We show here that bus-1 encodes a predicted acyltransferase expressed in rectal epithelial cells (K, F, and U), suggesting its involvement in regional surface modification. bus-1 reporter genes were used to show spatial regulation by hindgut developmental control genes: egl-38, mab-9, and mab-23. A bus-1::GFP reporter reveals the conspicuous rectal epithelial swelling induced by M. nematophilum. The C. briggsae ortholog of bus-1 exhibits conserved function and rectal expression, but it is expressed in vulval as well as rectal cells, correlated with pathogen adhesion to both vulval and rectal cells in this species. Another acyltransferase affecting bacterial adhesion, bus-18/acl-10, was also identified, which also shows strong rectal expression, but it is expressed in additional epithelial tissues and is required for general surface integrity.
Volume 237(12)
Pages 3762-76
Published 2008-12
DOI 10.1002/dvdy.21792
PMID 19035336
MeSH Actinomycetales / pathogenicity* Acyltransferases / chemistry Acyltransferases / genetics Acyltransferases / metabolism* Amino Acid Sequence Animals Caenorhabditis elegans / enzymology* Caenorhabditis elegans / genetics Caenorhabditis elegans / microbiology Caenorhabditis elegans Proteins / chemistry Caenorhabditis elegans Proteins / genetics Caenorhabditis elegans Proteins / metabolism* Epithelial Cells / enzymology Gene Expression Regulation, Enzymologic / genetics* Genes, Reporter / genetics Molecular Sequence Data Mutation / genetics Rectum / embryology Rectum / enzymology Sequence Alignment Sequence Homology, Amino Acid
IF 2.507
Times Cited 11
WOS Category ANATOMY & MORPHOLOGY DEVELOPMENTAL BIOLOGY
Resource
C.elegans tm1045 tm2164