RRC ID 42389
著者 Takashi M, Tsubaki S, Tsuzuki T, Duarte WR, Yamauchi M, Sato H.
タイトル Differential gene expression of collagen-binding small leucine-rich proteoglycans and lysyl hydroxylases, during mineralization by MC3T3-E1 cells cultured on titanium implant material.
ジャーナル Eur J Oral Sci
Abstract Titanium implants create a unique ultrastructure (composed of a collagenous zone with relatively disorganized fibril morphology) at the bone-implant interface. The objective of this study was to investigate the temporal mRNA expression patterns, using real-time polymerase chain reaction, of type I collagen (COLI) and regulators for collagen fibrillogenesis, collagen-binding small leucine-rich proteoglycans (SLRPs) and lysyl hydroxylases (LHs), during mineralization, by MC3T3-E1 cells cultured on titanium (Ti). Lysates of the cultures on Ti and on plastic wells (Pl) for 10-50 d were used for the quantification of calcium and mRNA. Although the onset of calcium accumulation in the cultures on Ti (30-40 d) was slower than that of cultures on Pl (20-30 d), the gene expression patterns during mineralization were similar in cells cultured on either material. COLI and fibromodulin were up-regulated just before the onset of mineralization and then down-regulated. Lumican and LH1 were up-regulated just before the onset of mineralization and then returned to the baseline level. Decorin and LH2 were up-regulated at the late mineralization stage. Biglycan was down-regulated once at the early mineralization stage and then returned to the original level. LH3 was maintained at a steady level throughout. This study suggests actual but distinct roles of SLRPs and LHs in the formation of a unique ultrastructure at the bone-implant interface.
巻・号 113(3)
ページ 225-31
公開日 2005-6-1
DOI 10.1111/j.1600-0722.2005.00208.x
PII EOS208
PMID 15953247
MeSH 3T3 Cells Animals Calcification, Physiologic / physiology* Calcium / analysis Chondroitin Sulfate Proteoglycans / analysis Chondroitin Sulfate Proteoglycans / genetics Collagen Type I / analysis* Collagen Type I / genetics Decorin Dental Implants Dental Materials* / chemistry Down-Regulation Extracellular Matrix Proteins / analysis Extracellular Matrix Proteins / genetics Fibromodulin Gene Expression Regulation / genetics Keratan Sulfate / analysis Keratan Sulfate / genetics Leucine / analysis* Leucine / genetics Lumican Mice Osteoblasts / metabolism Plastics / chemistry Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / analysis* Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / genetics Proteoglycans / analysis* Proteoglycans / genetics RNA, Messenger / analysis Titanium* / chemistry Transforming Growth Factor beta / antagonists & inhibitors Up-Regulation
IF 2.22
引用数 12
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)