RRC ID |
42389
|
著者 |
Takashi M, Tsubaki S, Tsuzuki T, Duarte WR, Yamauchi M, Sato H.
|
タイトル |
Differential gene expression of collagen-binding small leucine-rich proteoglycans and lysyl hydroxylases, during mineralization by MC3T3-E1 cells cultured on titanium implant material.
|
ジャーナル |
Eur J Oral Sci
|
Abstract |
Titanium implants create a unique ultrastructure (composed of a collagenous zone with relatively disorganized fibril morphology) at the bone-implant interface. The objective of this study was to investigate the temporal mRNA expression patterns, using real-time polymerase chain reaction, of type I collagen (COLI) and regulators for collagen fibrillogenesis, collagen-binding small leucine-rich proteoglycans (SLRPs) and lysyl hydroxylases (LHs), during mineralization, by MC3T3-E1 cells cultured on titanium (Ti). Lysates of the cultures on Ti and on plastic wells (Pl) for 10-50 d were used for the quantification of calcium and mRNA. Although the onset of calcium accumulation in the cultures on Ti (30-40 d) was slower than that of cultures on Pl (20-30 d), the gene expression patterns during mineralization were similar in cells cultured on either material. COLI and fibromodulin were up-regulated just before the onset of mineralization and then down-regulated. Lumican and LH1 were up-regulated just before the onset of mineralization and then returned to the baseline level. Decorin and LH2 were up-regulated at the late mineralization stage. Biglycan was down-regulated once at the early mineralization stage and then returned to the original level. LH3 was maintained at a steady level throughout. This study suggests actual but distinct roles of SLRPs and LHs in the formation of a unique ultrastructure at the bone-implant interface.
|
巻・号 |
113(3)
|
ページ |
225-31
|
公開日 |
2005-6-1
|
DOI |
10.1111/j.1600-0722.2005.00208.x
|
PII |
EOS208
|
PMID |
15953247
|
MeSH |
3T3 Cells
Animals
Calcification, Physiologic / physiology*
Calcium / analysis
Chondroitin Sulfate Proteoglycans / analysis
Chondroitin Sulfate Proteoglycans / genetics
Collagen Type I / analysis*
Collagen Type I / genetics
Decorin
Dental Implants
Dental Materials* / chemistry
Down-Regulation
Extracellular Matrix Proteins / analysis
Extracellular Matrix Proteins / genetics
Fibromodulin
Gene Expression Regulation / genetics
Keratan Sulfate / analysis
Keratan Sulfate / genetics
Leucine / analysis*
Leucine / genetics
Lumican
Mice
Osteoblasts / metabolism
Plastics / chemistry
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / analysis*
Procollagen-Lysine, 2-Oxoglutarate 5-Dioxygenase / genetics
Proteoglycans / analysis*
Proteoglycans / genetics
RNA, Messenger / analysis
Titanium* / chemistry
Transforming Growth Factor beta / antagonists & inhibitors
Up-Regulation
|
IF |
2.22
|
引用数 |
12
|
リソース情報 |
ヒト・動物細胞 |
MC3T3-E1(RCB1126) |