論文 - 詳細
| RRC ID | 42416 |
|---|---|
| 著者 | Okumura S, Saitoh H, Ishikawa T, Wasano N, Yamashita S, Kusumoto K, Akao T, Mizuki E, Ohba M, Inouye K. |
| タイトル | Identification of a novel cytotoxic protein, Cry45Aa, from Bacillus thuringiensis A1470 and its selective cytotoxic activity against various mammalian cell lines. |
| ジャーナル | J Agric Food Chem |
| Abstract |
Parasporal inclusion proteins produced by Bacillus thuringiensis strain A1470 exhibit strong cytotoxicity against human leukemic T cells when activated by protease treatment. One of the cytotoxic proteins was separated by anion exchange chromatography and gel filtration chromatography and designated Cry45Aa. Its gene was then expressed in recombinant Escherichia coli, in which the Cry45Aa precursor was accumulated in an inclusion body. It was solubilized in sodium carbonate buffer and processed with proteinase K, and cytotoxic activities of the protein against various mammalian cell lines were evaluated using the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide assay. The protein exhibited high cytotoxic activity against CACO-2, Sawano, MOLT-4, TCS, and HL60 cells and moderate activity against U-937 DE-4, PC12, and HepG2 cells. On the other hand, the EC50 values against Jurkat, K562, HeLa, A549, Vero, COS-7, NIH3T3, CHO, and four normal tissue cells (human primary hepatocyte cells, UtSMC, MRC-5, and normal T cells) were >2 microg/mL. |
| 巻・号 | 53(16) |
| ページ | 6313-8 |
| 公開日 | 2005-8-10 |
| DOI | 10.1021/jf0506129 |
| PMID | 16076112 |
| MeSH | Animals Antineoplastic Agents / pharmacology* Bacillus thuringiensis / chemistry* Bacterial Proteins / genetics Bacterial Proteins / isolation & purification Bacterial Proteins / pharmacology* Cell Death / drug effects Cell Line Cell Line, Tumor Cells, Cultured Escherichia coli / genetics Gene Expression Humans Recombinant Proteins / pharmacology |
| IF | 4.192 |
| 引用数 | 25 |
| WOS 分野 | AGRICULTURE, MULTIDISCIPLINARY CHEMISTRY, APPLIED FOOD SCIENCE & TECHNOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | MOLT-4(RCB0206) Jurkat(RCB0806) HL60(RCB0041) Sawano(RCB1152) Hep G2 HeLa(RCB0007) TCS(RCB0638) A549(RCB0098) MRC-5 CACO-2(RCB0988) COS-7(RCB0539) K562(RCB0027) CHO-K1(RCB0285) PC-12(RCB009) U-937 DE-4(RCB0435) Vero(RCB0001) NIH3T3 |