論文 - 詳細
| RRC ID | 42431 |
|---|---|
| 著者 | Tsurushima H, Yoshii Y, Leong KW, Ohno T. |
| タイトル | Targeted tumor cell death induced by autologous tumor-specific T lymphocyte recognition of wild-type p53-derived peptides. |
| ジャーナル | J Neurooncol |
| Abstract |
Autologous tumor-specific T lymphocyte (ATTL) lines were derived from the peripheral blood mononuclear cells (PBMC) of a healthy volunteer with human leukocyte antigen (HLA) -A*0201. These lines were achieved using interleukins -1beta, -2, -4, and -6 and the p53-based peptide from the 264-272 sequence of the wild-type p53 protein with a strong affinity against HLA-A*0201.;The frequencies of CD3+, CD4+, and CD8+ lymphocytes were 94-96%, 30-34%, and 69-74%, respectively. ATTLs killed most of the T2 cells pulsed with p53-derived peptide, but not against the T2 cells non-pulsed or pulsed with an irrelevant peptide. ATTLs also killed TKB-14 cells, which have been derived from human glioblastoma multiforme, and exhibited HLA-A*0201 molecule and immunohistochemical accumulation of p53 protein. These cytotoxic activities were inhibited by anti-CD3, anti-CD8, and anti-class I antibodies. These findings suggested that these ATTL lines might include CTL populations, which could recognize p53-derived peptide on HLA-A*0201 and the p53-based peptide may play as an antigen on HLA-A*0201. When tumor antigens would be more analyzed in the future, ATTL could be induced without the primary-cultured cells from tumor tissue and could be applied for cancer therapy. |
| 巻・号 | 76(2) |
| ページ | 99-104 |
| 公開日 | 2006-1-1 |
| DOI | 10.1007/s11060-005-4172-6 |
| PMID | 16132498 |
| MeSH | Antigens, Surface / immunology Brain Neoplasms / immunology* Brain Neoplasms / pathology* CD3 Complex / immunology CD4-Positive T-Lymphocytes / immunology CD8-Positive T-Lymphocytes / immunology Cell Death / physiology* Cell Line, Tumor Cells, Cultured Flow Cytometry HLA-A Antigens / immunology Humans Immunohistochemistry K562 Cells Monocytes / metabolism Peptides / immunology Phenotype T-Lymphocytes / immunology* Tumor Suppressor Protein p53 / immunology* |
| IF | 3.267 |
| 引用数 | 0 |
| WOS 分野 | CLINICAL NEUROLOGY ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| ヒト・動物細胞 | K562(RCB0027) U251(RCB0461) KG-1-C(RCB0270) |