RRC ID 42441
著者 Matsura T, Togawa A, Kai M, Nishida T, Nakada J, Ishibe Y, Kojo S, Yamamoto Y, Yamada K.
タイトル The presence of oxidized phosphatidylserine on Fas-mediated apoptotic cell surface.
ジャーナル Biochim Biophys Acta
Abstract A growing body of evidence suggests that phosphatidylserine (PS) oxidation is linked with its transmembrane migration from the inner to the outer leaflet of the plasma membrane during apoptosis. However, there is no direct evidence for the presence of oxidized PS (PSox) on the surface of cells undergoing apoptosis. The present study was performed to detect PSox externalized to the cell surface after Fas engagement in Jurkat cells. Treatment of Jurkat cells with anti-Fas antibody induced caspase-3 activation, chromatin condensation, PS externalization, generation of reactive oxygen species, intracellular glutathione depletion, disruption of mitochondrial transmembrane potential and release of cytochrome c from mitochondria. To determine externalized PS and phosphatidylethanolamine (PE), Jurkat cells were treated with anti-Fas antibody and then labeled with membrane-impermeable fluorescamine, a probe for visualizing lipids that contain primary amino groups. Their total lipids were extracted and subjected to two-dimensional high-performance thin-layer chromatography (HPTLC). The HPTLC plate was sprayed with N,N,N',N'-tetramethyl-p-phenylenediamine dihydrochloride to detect phospholipid hydroperoxides. PSox was present in small amounts within but not on the surface of normal cells. Treatment with anti-Fas antibody increased PSox within the cells and caused PSox to appear on the cell surface. In contrast, PE on the surface of Fas-ligated cells was not oxidized. Thus, the present study demonstrates for the first time the presence of PSox both within and on the surface of apoptotic cells.
巻・号 1736(3)
ページ 181-8
公開日 2005-10-1
DOI 10.1016/j.bbalip.2005.08.011
PII S1388-1981(05)00200-3
PMID 16168707
MeSH Antibodies, Monoclonal / pharmacology Apoptosis / physiology* Caspase 3 Caspases / metabolism Cell Membrane / drug effects Cell Membrane / metabolism* Cytochromes c / metabolism Glutathione / metabolism Humans Jurkat Cells Membrane Potentials / drug effects Mitochondria / drug effects Mitochondria / metabolism Oxidation-Reduction / drug effects Phosphatidylserines / metabolism* Reactive Oxygen Species / metabolism Receptors, Tumor Necrosis Factor / immunology Receptors, Tumor Necrosis Factor / physiology* T-Lymphocytes / drug effects T-Lymphocytes / metabolism fas Receptor
IF 3.411
引用数 29
WOS 分野 BIOPHYSICS BIOCHEMISTRY & MOLECULAR BIOLOGY CELL BIOLOGY
リソース情報
ヒト・動物細胞 Jurkat(RCB0806)