RRC ID 42453
Author Roybon L, Brundin P, Li JY.
Title Stromal cell-derived inducing activity does not promote dopaminergic differentiation, but enhances differentiation and proliferation of neural stem cell-derived astrocytes.
Journal Exp Neurol
Abstract Previous evidence has shown that stromal cell-derived inducing activity (SDIA), produced by the mouse PA6 stromal cell line, promotes dopaminergic differentiation of mouse, monkey and human embryonic stem cells in vitro. To examine whether PA6 stromal cells can enhance the yield of dopaminergic differentiation from neural progenitors, we generated neurospheres from embryonic day 11.5 (E11.5) (midbrain and forebrain) and E14.5 (ventral mesencephalon and cortex) rat embryos and allowed them to differentiate in co-culture with PA6 cells or poly-l-lysine/laminin-coated dishes. We observed that SDIA did not promote dopaminergic differentiation of E11.5 and E14.5 neurospheres but more prominently, enhanced astrocyte differentiation, cell survival and astrocyte proliferation. Our results suggest that PA6 cells do not have a general capacity to promote differentiation into dopaminergic neurons from all types of stem cells, but that they may specifically induce dopaminergic differentiation of highly uncommitted stem cells such as embryonic stem cells.
Volume 196(2)
Pages 373-80
Published 2005-12-1
DOI 10.1016/j.expneurol.2005.08.023
PII S0014-4886(05)00303-1
PMID 16199035
MeSH Analysis of Variance Animals Astrocytes / cytology* Brain / cytology* Bromodeoxyuridine / metabolism Cell Count / methods Cell Differentiation / physiology* Cell Proliferation* Cells, Cultured Coculture Techniques / methods Dopamine / metabolism Embryo, Mammalian Female Glial Fibrillary Acidic Protein / metabolism Immunohistochemistry / methods Neurons / physiology* Polylysine / pharmacology Pregnancy Rats Stromal Cells / cytology* Time Factors Tubulin / metabolism Tyrosine 3-Monooxygenase / metabolism
IF 4.691
Times Cited 13
Human and Animal Cells MC3T3-G2/PA6(RCB1127)