Reference - Detail
| RRC ID | 42483 |
|---|---|
| Author | Iwaoka S, Nakamura T, Takano S, Tsuchiya S, Aramaki Y. |
| Title | Cationic liposomes induce apoptosis through p38 MAP kinase-caspase-8-Bid pathway in macrophage-like RAW264.7 cells. |
| Journal | J Leukoc Biol |
| Abstract |
We have demonstrated that cationic liposomes composed of stearylamine (SA-liposomes) induce apoptosis in a variety of cells, but the mechanism responsible for the cellular death is not clear. In this paper, we investigated the signaling pathways implicated in SA-liposome-induced apoptosis in the macrophage-like cell line RAW264.7. Treatment with SA-liposomes caused the activation of mitogen-activated protein kinases (MAPKs), especially p38 and c-jun N-terminal kinase, and apoptosis was only inhibited upon the addition of a specific inhibitor for p38. N-acetylcysteine, a scavenger of reactive oxygen species (ROS), effectively inhibited the activation of p38 and cellular death, indicating that the activation induced by ROS is an initial step in the process of apoptosis triggered by SA-liposomes. Caspase-8 was activated by p38, and caspase-8-dependent cleavage of Bid was also observed. No down-regulation of bcl-2 expression, and no cleavage of Bax protein were observed. Taken together, our results suggest that apoptosis of RAW264.7 by SA-liposomes was mediated by the MAPK p38 and a caspase-8-dependent Bid-cleavage pathway. Moreover, we found that ROS can contribute intimately to the SA-liposome-induced cell death in RAW264.7. |
| Volume | 79(1) |
| Pages | 184-91 |
| Published | 2006-1-1 |
| DOI | 10.1189/jlb.0405181 |
| PII | jlb.0405181 |
| PMID | 16275897 |
| MeSH | Amines / chemistry Amines / pharmacology* Animals Apoptosis / drug effects* BH3 Interacting Domain Death Agonist Protein / metabolism Caspase 8 Caspases / metabolism Cell Line Down-Regulation / drug effects Free Radical Scavengers / pharmacology JNK Mitogen-Activated Protein Kinases / metabolism Liposomes / chemistry Liposomes / pharmacology* MAP Kinase Signaling System / drug effects* Mice Proto-Oncogene Proteins c-bcl-2 / metabolism Reactive Oxygen Species / metabolism Serine / analogs & derivatives Serine / pharmacology p38 Mitogen-Activated Protein Kinases / metabolism |
| IF | 3.757 |
| Times Cited | 71 |
| WOS Category | IMMUNOLOGY HEMATOLOGY CELL BIOLOGY |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | Patent(IFI CLAIMS) |
| Total number of mentions | 14 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Human and Animal Cells | RAW 264(RCB0535) |