論文 - 詳細
| RRC ID | 42495 |
|---|---|
| 著者 | Imura M, Yamashita S, Cai LY, Furuta J, Wakabayashi M, Yasugi T, Ushijima T. |
| タイトル | Methylation and expression analysis of 15 genes and three normally-methylated genes in 13 Ovarian cancer cell lines. |
| ジャーナル | Cancer Lett |
| Abstract |
Aberrant methylation of CpG islands (CGIs) in promoter regions of tumor-suppressor genes causes their silencing, and aberrant demethylation of normally methylated CGIs in promoter regions causes aberrant expression of cancer-testis antigens. Here, we comprehensively analyzed aberrant methylation of 15 genes and demethylation of three normally methylated genes in 13 ovarian cancer cell lines. RASSF1A was most frequently methylated (complete methylation in 7 and partial methylation in 4 cell lines), followed by ESR1 (5 and 2, respectively), FLNC (4 and 4), HAND1 (4 and 2), LOX (3 and 2), HRASLS (3 and 2), MGMT (3 and 0), CDKN2A (3 and 0), THBD (2 and 1), hMLH1 (2 and 0), CDH1 (1 and 1) and GSTP1 (1 and 0). hTERC and TIMP3 were only partially methylated in 7 and 2 cell lines, respectively. BRCA1 was not methylated at all. Aberrant demethylation of MAGE-A3, -B2 and -A1 was detected in 8, 4 and 3 cell lines, respectively. Gene expression was consistently absent in cell lines without unmethylated DNA molecules. Aberrant methylation was frequently observed in MCAS, RMUG-L (mucinous cell carcinomas), RTSG (poorly-differentiated carcinoma) and TYK-nu (undifferentiated carcinoma) while infrequent in HTOA, JHOS-2, and OV-90 (serous cell carcinomas). Aberrant demethylation was frequently observed in OV-90, OVK-18, and ES-2 cell lines. It was shown that aberrant methylation and demethylation were frequently observed in ovarian cancer cell lines, and these data will provide a basis for further epigenetic analysis in ovarian cancers. |
| 巻・号 | 241(2) |
| ページ | 213-20 |
| 公開日 | 2006-9-28 |
| DOI | 10.1016/j.canlet.2005.10.010 |
| PII | S0304-3835(05)00930-4 |
| PMID | 16303245 |
| MeSH | DNA Methylation* DNA, Neoplasm / genetics* Epigenesis, Genetic Female Gene Expression Profiling Gene Expression Regulation, Neoplastic / genetics* Genes, Neoplasm / genetics* Humans Ovarian Neoplasms / genetics* Promoter Regions, Genetic / genetics* Reverse Transcriptase Polymerase Chain Reaction Tumor Cells, Cultured Tumor Suppressor Proteins / genetics* |
| IF | 7.36 |
| 引用数 | 33 |
| WOS 分野 | ONCOLOGY |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | HTOA(RCB0692) JHOS-2(RCB1521) |