| RRC ID |
42509
|
| Author |
Takeda K, Yokoyama S, Aburatani H, Masuda T, Han F, Yoshizawa M, Yamaki N, Yamamoto H, Eguchi N, Urade Y, Shibahara S.
|
| Title |
Lipocalin-type prostaglandin D synthase as a melanocyte marker regulated by MITF.
|
| Journal |
Biochem Biophys Res Commun
|
| Abstract |
Microphthalmia-associated transcription factor (MITF) is responsible for differentiation of melanocytes. A recessive MITF mutant, black-eyed white Mitf(mi-bw) mouse, is characterized by white coat color and deafness, due to the lack of melanocytes in the skin and inner ears. By cDNA microarray analysis, we have identified lipocalin-type prostaglandin D synthase (L-PGDS), whose mRNA is undetectable in the homozygous Mitf(mi-bw) skin. Immunohistochemical analysis of wild-type mice identified the specific expression of L-PGDS in follicular melanocytes. L-PGDS mRNA is expressed in B16 mouse melanoma cells, but undetectable in human melanoma cell lines. RNA interference analysis against MITF suggests that L-PGDS expression is dependent on MITF in B16 melanoma cells. Furthermore, we have provided evidence that MITF is involved in the melanocyte lineage-specific transcription of the mouse L-PGDS gene. Thus, L-PGDS represents a newly identified melanocyte marker. MITF may modulate the production of prostaglandin D(2) by activating the L-PGDS gene in melanocytes.
|
| Volume |
339(4)
|
| Pages |
1098-106
|
| Published |
2006-1-27
|
| DOI |
10.1016/j.bbrc.2005.11.125
|
| PII |
S0006-291X(05)02629-X
|
| PMID |
16337607
|
| MeSH |
Animals
Biomarkers / metabolism
Biomarkers, Tumor / metabolism*
Cells, Cultured
Gene Expression Profiling
Intramolecular Oxidoreductases / metabolism*
Lipocalins
Melanocytes / metabolism*
Melanoma / metabolism*
Mice
Mice, Inbred BALB C
Mice, Inbred C3H
Microphthalmia-Associated Transcription Factor / metabolism*
Retinal Ganglion Cells / metabolism*
Skin / metabolism*
Tissue Distribution
|
| IF |
2.985
|
| Times Cited |
26
|
|
WOS Category
|
BIOPHYSICS
BIOCHEMISTRY & MOLECULAR BIOLOGY
|
| Resource |
| Human and Animal Cells |
HMV-II(RCB0777) |
