Reference - Detail
| RRC ID | 42526 |
|---|---|
| Author | Kobayashi A, Hirano F, Makino I. |
| Title | The inhibitory effect of bisphosphonates on glucocorticoid-induced RANKL expression in human cells. |
| Journal | Scand J Rheumatol |
| Abstract |
OBJECTIVE:RANKL is known to play an important role in activating osteoclasts and advancing the progress of osteoporosis. However, little is known about the effect of bisphosphonates on glucocorticoid-induced RANKL expression in human cells. Our study was intended to clarify effects of bisphosphonates on glucocorticoid-induced RANKL expression in human cells. METHODS:Human T lymphoblastic cell line Jurkat and human osteosarcoma cell line MG-63 were used for the following experiments. RANKL expression in two cell lines was measured using reverse transcription polymerase chain reaction (RT-PCR) analysis and enzyme immunoassay (EIA). Luciferase assays using pGRE-Luc were also performed. RESULTS:In Jurkat and MG-63 cells, dexamethasone induced expression of soluble RANKL (sRANKL) protein in supernatants and RANKL mRNA in cells. Moreover, bisphosphonates, but not cyclooxygenase inhibitors, repressed dexamethasone-induced sRANKL protein production. By contrast, glucocorticoid receptor-driven transcriptional activity was not inhibited by bisphosphonates. CONCLUSION:Glucocorticoid induced RANKL expression in human cells derived from T lymphocytes and osteoblasts. Bisphosphonates inhibited glucocorticoid-induced RANKL expression, suggesting that these effects might be a new therapeutic mechanism for bisphosphonates. |
| Volume | 34(6) |
| Pages | 480-4 |
| Published | 2005-1-1 |
| DOI | 10.1080/03009740510026788 |
| PII | P414M54P635Q8103 |
| PMID | 16393773 |
| MeSH | Anti-Inflammatory Agents / pharmacology Bone Density Conservation Agents / pharmacology* Carrier Proteins / genetics* Carrier Proteins / metabolism Dexamethasone / pharmacology* Diphosphonates / pharmacology* Gene Expression / drug effects Glucocorticoids / pharmacology* Humans Jurkat Cells / drug effects Jurkat Cells / metabolism Membrane Glycoproteins / genetics* Membrane Glycoproteins / metabolism Osteoblasts / drug effects Osteoblasts / metabolism Osteosarcoma / drug therapy Osteosarcoma / metabolism RANK Ligand RNA, Messenger / metabolism Receptor Activator of Nuclear Factor-kappa B Reverse Transcriptase Polymerase Chain Reaction T-Lymphocytes / drug effects T-Lymphocytes / metabolism |
| IF | 3.025 |
| Times Cited | 13 |
| WOS Category | RHEUMATOLOGY |
| Resource | |
| Human and Animal Cells | MG‐63(RCB1890) |