論文 - 詳細
| RRC ID | 42563 |
|---|---|
| 著者 | Elloumi I, Kobayashi R, Funabashi H, Mie M, Kobatake E. |
| タイトル | Construction of epidermal growth factor fusion protein with cell adhesive activity. |
| ジャーナル | Biomaterials |
| Abstract |
A novel protein for controlling cellular functions was constructed by combining functional units of various proteins. The Arg-Gly-Asp (RGD) sequence functioning as a cell adhesive function, an epidermal growth factor (EGF) as a cell growth function, and a hydrophobic sequence (E12) as an efficient assembling function, were combined and incorporated into one molecule. The fusion protein, designated ERE-EGF, was produced in Escherichia coli and purified with affinity chromatography using a His-tag. The ERE-EGF coated on an unmodified hydrophobic surface of a cell-culture plate (through the hydrophobic E12 moiety) retained both cell adhesive activity (through the RGD sequence) and cell growth activity (through the EGF moiety). |
| 巻・号 | 27(18) |
| ページ | 3451-8 |
| 公開日 | 2006-6-1 |
| DOI | 10.1016/j.biomaterials.2006.02.003 |
| PII | S0142-9612(06)00121-9 |
| PMID | 16497372 |
| MeSH | Amino Acid Sequence Cell Adhesion / drug effects* Escherichia coli / genetics Hydrophobic and Hydrophilic Interactions Molecular Sequence Data Oligopeptides / chemistry Oligopeptides / pharmacology Recombinant Fusion Proteins / chemistry* Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / pharmacology* Tumor Cells, Cultured |
| IF | 10.317 |
| 引用数 | 45 |
| WOS 分野 | ENGINEERING, BIOMEDICAL MATERIALS SCIENCE, BIOMATERIALS |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 2 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | A549(RCB0098) |